Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8

Using a ChIP-cloning technique, we identified a Zinc finger protein 804a (Zfp804a) as one of the putative Hoxc8 downstream target genes. We confirmed binding of Hoxc8 to an intronic region of Zfp804a by ChIP-PCR in F9 cells as well as in mouse embryos. Hoxc8 upregulated Zfp804a mRNA levels and augme...

Descripción completa

Detalles Bibliográficos
Autores principales: Chung, Hyun Joo, Lee, Ji-Yeon, Deocaris, Custer C., Min, Hyehyun, Kim, Sang Hoon, Kim, Myoung Hee
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876248/
https://www.ncbi.nlm.nih.gov/pubmed/20508826
http://dx.doi.org/10.1155/2010/231708
_version_ 1782181683743686656
author Chung, Hyun Joo
Lee, Ji-Yeon
Deocaris, Custer C.
Min, Hyehyun
Kim, Sang Hoon
Kim, Myoung Hee
author_facet Chung, Hyun Joo
Lee, Ji-Yeon
Deocaris, Custer C.
Min, Hyehyun
Kim, Sang Hoon
Kim, Myoung Hee
author_sort Chung, Hyun Joo
collection PubMed
description Using a ChIP-cloning technique, we identified a Zinc finger protein 804a (Zfp804a) as one of the putative Hoxc8 downstream target genes. We confirmed binding of Hoxc8 to an intronic region of Zfp804a by ChIP-PCR in F9 cells as well as in mouse embryos. Hoxc8 upregulated Zfp804a mRNA levels and augmented minimal promoter activity in vitro. In E11.5 mouse embryos, Zfp804a and Hoxc8 were coexpressed. Recent genome-wide studies identified Zfp804a (or ZNF804A in humans) as a plausible marker for schizophrenia, leading us to hypothesize that this embryogenic regulatory control might also exert influence in development of complex traits such as psychosis.
format Text
id pubmed-2876248
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-28762482010-05-27 Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8 Chung, Hyun Joo Lee, Ji-Yeon Deocaris, Custer C. Min, Hyehyun Kim, Sang Hoon Kim, Myoung Hee J Biomed Biotechnol Research Article Using a ChIP-cloning technique, we identified a Zinc finger protein 804a (Zfp804a) as one of the putative Hoxc8 downstream target genes. We confirmed binding of Hoxc8 to an intronic region of Zfp804a by ChIP-PCR in F9 cells as well as in mouse embryos. Hoxc8 upregulated Zfp804a mRNA levels and augmented minimal promoter activity in vitro. In E11.5 mouse embryos, Zfp804a and Hoxc8 were coexpressed. Recent genome-wide studies identified Zfp804a (or ZNF804A in humans) as a plausible marker for schizophrenia, leading us to hypothesize that this embryogenic regulatory control might also exert influence in development of complex traits such as psychosis. Hindawi Publishing Corporation 2010 2010-05-25 /pmc/articles/PMC2876248/ /pubmed/20508826 http://dx.doi.org/10.1155/2010/231708 Text en Copyright © 2010 Hyun Joo Chung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chung, Hyun Joo
Lee, Ji-Yeon
Deocaris, Custer C.
Min, Hyehyun
Kim, Sang Hoon
Kim, Myoung Hee
Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8
title Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8
title_full Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8
title_fullStr Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8
title_full_unstemmed Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8
title_short Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8
title_sort mouse homologue of the schizophrenia susceptibility gene znf804a as a target of hoxc8
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876248/
https://www.ncbi.nlm.nih.gov/pubmed/20508826
http://dx.doi.org/10.1155/2010/231708
work_keys_str_mv AT chunghyunjoo mousehomologueoftheschizophreniasusceptibilitygeneznf804aasatargetofhoxc8
AT leejiyeon mousehomologueoftheschizophreniasusceptibilitygeneznf804aasatargetofhoxc8
AT deocariscusterc mousehomologueoftheschizophreniasusceptibilitygeneznf804aasatargetofhoxc8
AT minhyehyun mousehomologueoftheschizophreniasusceptibilitygeneznf804aasatargetofhoxc8
AT kimsanghoon mousehomologueoftheschizophreniasusceptibilitygeneznf804aasatargetofhoxc8
AT kimmyounghee mousehomologueoftheschizophreniasusceptibilitygeneznf804aasatargetofhoxc8

Ejemplares similares