Enhanced Subtyping Scheme for Salmonella Enteritidis

To improve pulsed-field gel electrophoresis–based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the...

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Detalles Bibliográficos
Autores principales: Zheng, Jie, Keys, Christine E., Zhao, Shaohua, Meng, Jianghong, Brown, Eric W.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2007
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876743/
https://www.ncbi.nlm.nih.gov/pubmed/18258051
http://dx.doi.org/10.3201/eid1312.070185
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author Zheng, Jie
Keys, Christine E.
Zhao, Shaohua
Meng, Jianghong
Brown, Eric W.
author_facet Zheng, Jie
Keys, Christine E.
Zhao, Shaohua
Meng, Jianghong
Brown, Eric W.
author_sort Zheng, Jie
collection PubMed
description To improve pulsed-field gel electrophoresis–based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the Simpson diversity index, supported this 3-enzyme combination for improved differentiation of S. Enteritidis.
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spelling pubmed-28767432010-06-01 Enhanced Subtyping Scheme for Salmonella Enteritidis Zheng, Jie Keys, Christine E. Zhao, Shaohua Meng, Jianghong Brown, Eric W. Emerg Infect Dis Dispatch To improve pulsed-field gel electrophoresis–based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the Simpson diversity index, supported this 3-enzyme combination for improved differentiation of S. Enteritidis. Centers for Disease Control and Prevention 2007-12 /pmc/articles/PMC2876743/ /pubmed/18258051 http://dx.doi.org/10.3201/eid1312.070185 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Zheng, Jie
Keys, Christine E.
Zhao, Shaohua
Meng, Jianghong
Brown, Eric W.
Enhanced Subtyping Scheme for Salmonella Enteritidis
title Enhanced Subtyping Scheme for Salmonella Enteritidis
title_full Enhanced Subtyping Scheme for Salmonella Enteritidis
title_fullStr Enhanced Subtyping Scheme for Salmonella Enteritidis
title_full_unstemmed Enhanced Subtyping Scheme for Salmonella Enteritidis
title_short Enhanced Subtyping Scheme for Salmonella Enteritidis
title_sort enhanced subtyping scheme for salmonella enteritidis
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876743/
https://www.ncbi.nlm.nih.gov/pubmed/18258051
http://dx.doi.org/10.3201/eid1312.070185
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AT keyschristinee enhancedsubtypingschemeforsalmonellaenteritidis
AT zhaoshaohua enhancedsubtypingschemeforsalmonellaenteritidis
AT mengjianghong enhancedsubtypingschemeforsalmonellaenteritidis
AT brownericw enhancedsubtypingschemeforsalmonellaenteritidis

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