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Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid

BACKGROUND AND PURPOSE: Experimental models of osteoporosis in rabbits are useful to investigate anabolic agents because rabbits have an active Haversian remodeling and achieve skeletal maturiaty quickly. In this study, an experimental model of osteoporosis in rabbits induced by a combination of ova...

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Autores principales: Baofeng, Li, Zhi, Yuan, Bei, Chen, Guolin, Meng, Qingshui, Yin, Jian, Liu
Formato: Texto
Lenguaje:English
Publicado: Informa Healthcare 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876847/
https://www.ncbi.nlm.nih.gov/pubmed/20446884
http://dx.doi.org/10.3109/17453674.2010.483986
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author Baofeng, Li
Zhi, Yuan
Bei, Chen
Guolin, Meng
Qingshui, Yin
Jian, Liu
author_facet Baofeng, Li
Zhi, Yuan
Bei, Chen
Guolin, Meng
Qingshui, Yin
Jian, Liu
author_sort Baofeng, Li
collection PubMed
description BACKGROUND AND PURPOSE: Experimental models of osteoporosis in rabbits are useful to investigate anabolic agents because rabbits have an active Haversian remodeling and achieve skeletal maturiaty quickly. In this study, an experimental model of osteoporosis in rabbits induced by a combination of ovariectomy and glucocorticoid was characterized to provide a useful model for prevention and therapy of osteoporosis. METHODS: 32 skeletally mature female rabbits were divided randomly into 4 groups: sham control, bilateral ovariectomy (OVX), 1mg/kg/day methylprednisolone (MP) for 8 weeks, and OVX in combination with MP. All rabbits were killed 10 weeks after surgery. Bone mineral density (BMD) of lumbar spine was measured by dual-energy X-ray absorptiometry at baseline, and at 6 and 10 weeks postoperatively. Bone microarchitecture and mechanical properties of lumbar vertebrae were investigated by high-resolution micro-computed tomography and compression test, respectively, after killing. RESULTS: Mean BMD of the OVX+MP group at 10 weeks was reduced by about 36% from baseline (p < 0.001). Bone microarchitecture of lumbar vertebrae in the OVX+MP group indicated osteoporosis-associated deterioration. There was a statistically significant reduction in maximum load, stiffness, and energy absorption capacity of lumbar vertebrae in the OVX+MP group, but not in the OVX group, compared to the sham control. In the MP group, BMD and some microarchitecture parameters such as trabecular thickness and bone volume fraction were reduced. The mechanical properties were not statistically significantly different from those in the sham control group, however, although a negative trend was observed. INTERPRETATION: Osteopenia can be induced experimentally in rabbits through a combination of OVX and MP, and can be evaluated by BMD, bone microstructure, and mechanical parameters.
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spelling pubmed-28768472010-09-03 Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid Baofeng, Li Zhi, Yuan Bei, Chen Guolin, Meng Qingshui, Yin Jian, Liu Acta Orthop Research Article BACKGROUND AND PURPOSE: Experimental models of osteoporosis in rabbits are useful to investigate anabolic agents because rabbits have an active Haversian remodeling and achieve skeletal maturiaty quickly. In this study, an experimental model of osteoporosis in rabbits induced by a combination of ovariectomy and glucocorticoid was characterized to provide a useful model for prevention and therapy of osteoporosis. METHODS: 32 skeletally mature female rabbits were divided randomly into 4 groups: sham control, bilateral ovariectomy (OVX), 1mg/kg/day methylprednisolone (MP) for 8 weeks, and OVX in combination with MP. All rabbits were killed 10 weeks after surgery. Bone mineral density (BMD) of lumbar spine was measured by dual-energy X-ray absorptiometry at baseline, and at 6 and 10 weeks postoperatively. Bone microarchitecture and mechanical properties of lumbar vertebrae were investigated by high-resolution micro-computed tomography and compression test, respectively, after killing. RESULTS: Mean BMD of the OVX+MP group at 10 weeks was reduced by about 36% from baseline (p < 0.001). Bone microarchitecture of lumbar vertebrae in the OVX+MP group indicated osteoporosis-associated deterioration. There was a statistically significant reduction in maximum load, stiffness, and energy absorption capacity of lumbar vertebrae in the OVX+MP group, but not in the OVX group, compared to the sham control. In the MP group, BMD and some microarchitecture parameters such as trabecular thickness and bone volume fraction were reduced. The mechanical properties were not statistically significantly different from those in the sham control group, however, although a negative trend was observed. INTERPRETATION: Osteopenia can be induced experimentally in rabbits through a combination of OVX and MP, and can be evaluated by BMD, bone microstructure, and mechanical parameters. Informa Healthcare 2010-06 2010-05-21 /pmc/articles/PMC2876847/ /pubmed/20446884 http://dx.doi.org/10.3109/17453674.2010.483986 Text en Copyright: © Nordic Orthopedic Federation http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.
spellingShingle Research Article
Baofeng, Li
Zhi, Yuan
Bei, Chen
Guolin, Meng
Qingshui, Yin
Jian, Liu
Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
title Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
title_full Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
title_fullStr Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
title_full_unstemmed Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
title_short Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
title_sort characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876847/
https://www.ncbi.nlm.nih.gov/pubmed/20446884
http://dx.doi.org/10.3109/17453674.2010.483986
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