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Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion
Amassments of heterochromatin in somatic cells occur in close contact with the nuclear envelope (NE) but are gapped by channel- and cone-like zones that appear largely free of heterochromatin and associated with the nuclear pore complexes (NPCs). To identify proteins involved in forming such heteroc...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876962/ https://www.ncbi.nlm.nih.gov/pubmed/20407419 http://dx.doi.org/10.1038/emboj.2010.54 |
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author | Krull, Sandra Dörries, Julia Boysen, Björn Reidenbach, Sonja Magnius, Lars Norder, Helene Thyberg, Johan Cordes, Volker C |
author_facet | Krull, Sandra Dörries, Julia Boysen, Björn Reidenbach, Sonja Magnius, Lars Norder, Helene Thyberg, Johan Cordes, Volker C |
author_sort | Krull, Sandra |
collection | PubMed |
description | Amassments of heterochromatin in somatic cells occur in close contact with the nuclear envelope (NE) but are gapped by channel- and cone-like zones that appear largely free of heterochromatin and associated with the nuclear pore complexes (NPCs). To identify proteins involved in forming such heterochromatin exclusion zones (HEZs), we used a cell culture model in which chromatin condensation induced by poliovirus (PV) infection revealed HEZs resembling those in normal tissue cells. HEZ occurrence depended on the NPC-associated protein Tpr and its large coiled coil-forming domain. RNAi-mediated loss of Tpr allowed condensing chromatin to occur all along the NE's nuclear surface, resulting in HEZs no longer being established and NPCs covered by heterochromatin. These results assign a central function to Tpr as a determinant of perinuclear organization, with a direct role in forming a morphologically distinct nuclear sub-compartment and delimiting heterochromatin distribution. |
format | Text |
id | pubmed-2876962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-28769622010-06-02 Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion Krull, Sandra Dörries, Julia Boysen, Björn Reidenbach, Sonja Magnius, Lars Norder, Helene Thyberg, Johan Cordes, Volker C EMBO J Article Amassments of heterochromatin in somatic cells occur in close contact with the nuclear envelope (NE) but are gapped by channel- and cone-like zones that appear largely free of heterochromatin and associated with the nuclear pore complexes (NPCs). To identify proteins involved in forming such heterochromatin exclusion zones (HEZs), we used a cell culture model in which chromatin condensation induced by poliovirus (PV) infection revealed HEZs resembling those in normal tissue cells. HEZ occurrence depended on the NPC-associated protein Tpr and its large coiled coil-forming domain. RNAi-mediated loss of Tpr allowed condensing chromatin to occur all along the NE's nuclear surface, resulting in HEZs no longer being established and NPCs covered by heterochromatin. These results assign a central function to Tpr as a determinant of perinuclear organization, with a direct role in forming a morphologically distinct nuclear sub-compartment and delimiting heterochromatin distribution. Nature Publishing Group 2010-05-19 2010-04-20 /pmc/articles/PMC2876962/ /pubmed/20407419 http://dx.doi.org/10.1038/emboj.2010.54 Text en Copyright © 2010, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission. |
spellingShingle | Article Krull, Sandra Dörries, Julia Boysen, Björn Reidenbach, Sonja Magnius, Lars Norder, Helene Thyberg, Johan Cordes, Volker C Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion |
title | Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion |
title_full | Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion |
title_fullStr | Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion |
title_full_unstemmed | Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion |
title_short | Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion |
title_sort | protein tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876962/ https://www.ncbi.nlm.nih.gov/pubmed/20407419 http://dx.doi.org/10.1038/emboj.2010.54 |
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