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Resistance of Dictyostelium discoideum membranes to saponin permeabilization
BACKGROUND: Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae. FINDINGS: We show that saponin, contrary to Triton X-100 or alcohol, permeabiliz...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877058/ https://www.ncbi.nlm.nih.gov/pubmed/20426861 http://dx.doi.org/10.1186/1756-0500-3-120 |