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Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival

BACKGROUND: Recent attention has been given to the relationships between diet, longevity, aging and resistance to various forms of stress. Flies do not simply ingest calories. They sense different concentrations of carbohydrate and protein macronutrients and they modify their feeding behavior in res...

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Autores principales: Vigne, Paul, Frelin, Christian
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877658/
https://www.ncbi.nlm.nih.gov/pubmed/20465825
http://dx.doi.org/10.1186/1472-6793-10-8
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author Vigne, Paul
Frelin, Christian
author_facet Vigne, Paul
Frelin, Christian
author_sort Vigne, Paul
collection PubMed
description BACKGROUND: Recent attention has been given to the relationships between diet, longevity, aging and resistance to various forms of stress. Flies do not simply ingest calories. They sense different concentrations of carbohydrate and protein macronutrients and they modify their feeding behavior in response to changes in dietary conditions. Chronic hypoxia is a major consequence of cardiovascular diseases. Dietary proteins have recently been shown to decrease the survival of chronically hypoxic Drosophila. Whether flies modify their feeding behavior in response to hypoxia is not currently known. This study uses the recently developed capillary feeding assay to analyze the feeding behavior of normoxic and chronically hypoxic Drosophila melanogaster. RESULTS: The intakes rates of sucrose and yeast by normoxic or chronically hypoxic flies (5% O(2)) were analyzed under self selecting and "no choice" conditions. Chronically hypoxic flies fed on pure yeast diets or mixed diets under self selection conditions stopped feeding on yeast. Flies fed on mixed diets under "no choice" conditions reduced their food intakes. Hypoxia did not modify the adaptation of flies to diluted diets or to imbalanced diets. Mortality was assessed in parallel experiments. Dietary yeast had two distinct effects on hypoxic flies (i) a repellent action which eventually led to starvation and which was best observed in the absence of dietary sucrose and (ii) a toxic action which led to premature death. Finally we determined that hypoxic survivals were correlated to the intakes of sucrose, which suggested that dietary yeast killed flies by reducing their intake of sucrose. The feeding preferences of adult Drosophila were insensitive to NO scavengers, NO donor molecules and inhibitors of phosphodiesterases which are active on Drosophila larvae. CONCLUSION: Chronically hypoxic flies modify their feeding behavior. They avoid dietary yeast which appears to be toxic. Hypoxic survival is dependent on a source of exogenous sucrose. Ultimately, dietary yeast reduces hypoxic survival by reducing the intake of sucrose. The results highlight the importance of behavioral mechanisms in the responses of Drosophila to chronic hypoxic conditions.
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spelling pubmed-28776582010-05-27 Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival Vigne, Paul Frelin, Christian BMC Physiol Research article BACKGROUND: Recent attention has been given to the relationships between diet, longevity, aging and resistance to various forms of stress. Flies do not simply ingest calories. They sense different concentrations of carbohydrate and protein macronutrients and they modify their feeding behavior in response to changes in dietary conditions. Chronic hypoxia is a major consequence of cardiovascular diseases. Dietary proteins have recently been shown to decrease the survival of chronically hypoxic Drosophila. Whether flies modify their feeding behavior in response to hypoxia is not currently known. This study uses the recently developed capillary feeding assay to analyze the feeding behavior of normoxic and chronically hypoxic Drosophila melanogaster. RESULTS: The intakes rates of sucrose and yeast by normoxic or chronically hypoxic flies (5% O(2)) were analyzed under self selecting and "no choice" conditions. Chronically hypoxic flies fed on pure yeast diets or mixed diets under self selection conditions stopped feeding on yeast. Flies fed on mixed diets under "no choice" conditions reduced their food intakes. Hypoxia did not modify the adaptation of flies to diluted diets or to imbalanced diets. Mortality was assessed in parallel experiments. Dietary yeast had two distinct effects on hypoxic flies (i) a repellent action which eventually led to starvation and which was best observed in the absence of dietary sucrose and (ii) a toxic action which led to premature death. Finally we determined that hypoxic survivals were correlated to the intakes of sucrose, which suggested that dietary yeast killed flies by reducing their intake of sucrose. The feeding preferences of adult Drosophila were insensitive to NO scavengers, NO donor molecules and inhibitors of phosphodiesterases which are active on Drosophila larvae. CONCLUSION: Chronically hypoxic flies modify their feeding behavior. They avoid dietary yeast which appears to be toxic. Hypoxic survival is dependent on a source of exogenous sucrose. Ultimately, dietary yeast reduces hypoxic survival by reducing the intake of sucrose. The results highlight the importance of behavioral mechanisms in the responses of Drosophila to chronic hypoxic conditions. BioMed Central 2010-05-13 /pmc/articles/PMC2877658/ /pubmed/20465825 http://dx.doi.org/10.1186/1472-6793-10-8 Text en Copyright ©2010 Vigne and Frelin; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Vigne, Paul
Frelin, Christian
Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival
title Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival
title_full Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival
title_fullStr Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival
title_full_unstemmed Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival
title_short Hypoxia modifies the feeding preferences of Drosophila. Consequences for diet dependent hypoxic survival
title_sort hypoxia modifies the feeding preferences of drosophila. consequences for diet dependent hypoxic survival
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877658/
https://www.ncbi.nlm.nih.gov/pubmed/20465825
http://dx.doi.org/10.1186/1472-6793-10-8
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