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Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging

BACKGROUND: Dental pulp stem cells (DPSCs) can be driven into odontoblast, osteoblast, and chondrocyte lineages in different inductive media. However, the differentiation potential of naive DPSCs after serial passaging in the routine culture system has not been fully elucidated. RESULTS: DPSCs were...

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Autores principales: Yu, Jinhua, He, Huixia, Tang, Chunbo, Zhang, Guangdong, Li, Yuanfei, Wang, Ruoning, Shi, Junnan, Jin, Yan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877667/
https://www.ncbi.nlm.nih.gov/pubmed/20459680
http://dx.doi.org/10.1186/1471-2121-11-32
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author Yu, Jinhua
He, Huixia
Tang, Chunbo
Zhang, Guangdong
Li, Yuanfei
Wang, Ruoning
Shi, Junnan
Jin, Yan
author_facet Yu, Jinhua
He, Huixia
Tang, Chunbo
Zhang, Guangdong
Li, Yuanfei
Wang, Ruoning
Shi, Junnan
Jin, Yan
author_sort Yu, Jinhua
collection PubMed
description BACKGROUND: Dental pulp stem cells (DPSCs) can be driven into odontoblast, osteoblast, and chondrocyte lineages in different inductive media. However, the differentiation potential of naive DPSCs after serial passaging in the routine culture system has not been fully elucidated. RESULTS: DPSCs were isolated from human/rat dental pulps by the magnetic activated cell sorting based on STRO-1 expression, cultured and passaged in the conventional culture media. The biological features of STRO-1(+ )DPSCs at the 1(st )and 9(th )passages were investigated. During the long-term passage, the proliferation ability of human STRO-1(+ )DPSCs was downregulated as indicated by the growth kinetics. When compared with STRO-1(+ )DPSCs at the 1(st )passage (DPSC-P1), the expression of mature osteoblast-specific genes/proteins (alkaline phosphatase, bone sialoprotein, osterix, and osteopontin), odontoblast-specific gene/protein (dentin sialophosphoprotein and dentin sialoprotein), and chondrocyte-specific gene/protein (type II collagen) was significantly upregulated in human STRO-1(+ )DPSCs at the 9(th )passage (DPSC-P9). Furthermore, human DPSC-P9 cells in the mineralization-inducing media presented higher levels of alkaline phosphatase at day 3 and day 7 respectively, and produced more mineralized matrix than DPSC-P9 cells at day 14. In vivo transplantation results showed that rat DPSC-P1 cell pellets developed into dentin, bone and cartilage structures respectively, while DPSC-P9 cells can only generate bone tissues. CONCLUSIONS: These findings suggest that STRO-1(+ )DPSCs consist of several interrelated subpopulations which can spontaneously differentiate into odontoblasts, osteoblasts, and chondrocytes. The differentiation capacity of these DPSCs changes during cell passaging, and DPSCs at the 9(th )passage restrict their differentiation potential to the osteoblast lineage in vivo.
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spelling pubmed-28776672010-05-27 Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging Yu, Jinhua He, Huixia Tang, Chunbo Zhang, Guangdong Li, Yuanfei Wang, Ruoning Shi, Junnan Jin, Yan BMC Cell Biol Research article BACKGROUND: Dental pulp stem cells (DPSCs) can be driven into odontoblast, osteoblast, and chondrocyte lineages in different inductive media. However, the differentiation potential of naive DPSCs after serial passaging in the routine culture system has not been fully elucidated. RESULTS: DPSCs were isolated from human/rat dental pulps by the magnetic activated cell sorting based on STRO-1 expression, cultured and passaged in the conventional culture media. The biological features of STRO-1(+ )DPSCs at the 1(st )and 9(th )passages were investigated. During the long-term passage, the proliferation ability of human STRO-1(+ )DPSCs was downregulated as indicated by the growth kinetics. When compared with STRO-1(+ )DPSCs at the 1(st )passage (DPSC-P1), the expression of mature osteoblast-specific genes/proteins (alkaline phosphatase, bone sialoprotein, osterix, and osteopontin), odontoblast-specific gene/protein (dentin sialophosphoprotein and dentin sialoprotein), and chondrocyte-specific gene/protein (type II collagen) was significantly upregulated in human STRO-1(+ )DPSCs at the 9(th )passage (DPSC-P9). Furthermore, human DPSC-P9 cells in the mineralization-inducing media presented higher levels of alkaline phosphatase at day 3 and day 7 respectively, and produced more mineralized matrix than DPSC-P9 cells at day 14. In vivo transplantation results showed that rat DPSC-P1 cell pellets developed into dentin, bone and cartilage structures respectively, while DPSC-P9 cells can only generate bone tissues. CONCLUSIONS: These findings suggest that STRO-1(+ )DPSCs consist of several interrelated subpopulations which can spontaneously differentiate into odontoblasts, osteoblasts, and chondrocytes. The differentiation capacity of these DPSCs changes during cell passaging, and DPSCs at the 9(th )passage restrict their differentiation potential to the osteoblast lineage in vivo. BioMed Central 2010-05-08 /pmc/articles/PMC2877667/ /pubmed/20459680 http://dx.doi.org/10.1186/1471-2121-11-32 Text en Copyright ©2010 Yu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Yu, Jinhua
He, Huixia
Tang, Chunbo
Zhang, Guangdong
Li, Yuanfei
Wang, Ruoning
Shi, Junnan
Jin, Yan
Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging
title Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging
title_full Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging
title_fullStr Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging
title_full_unstemmed Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging
title_short Differentiation potential of STRO-1(+ )dental pulp stem cells changes during cell passaging
title_sort differentiation potential of stro-1(+ )dental pulp stem cells changes during cell passaging
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877667/
https://www.ncbi.nlm.nih.gov/pubmed/20459680
http://dx.doi.org/10.1186/1471-2121-11-32
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