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NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors

NK and γδ T cells can eliminate tumor cells in many experimental models, but their effect on the development of tumors caused by virus infections in vivo is not known. Polyomavirus (PyV) induces tumors in neonatally infected mice of susceptible strains and in adult mice with certain immune deficienc...

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Autores principales: Mishra, Rabinarayan, Chen, Alex T., Welsh, Raymond M., Szomolanyi-Tsuda, Eva
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877738/
https://www.ncbi.nlm.nih.gov/pubmed/20523894
http://dx.doi.org/10.1371/journal.ppat.1000924
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author Mishra, Rabinarayan
Chen, Alex T.
Welsh, Raymond M.
Szomolanyi-Tsuda, Eva
author_facet Mishra, Rabinarayan
Chen, Alex T.
Welsh, Raymond M.
Szomolanyi-Tsuda, Eva
author_sort Mishra, Rabinarayan
collection PubMed
description NK and γδ T cells can eliminate tumor cells in many experimental models, but their effect on the development of tumors caused by virus infections in vivo is not known. Polyomavirus (PyV) induces tumors in neonatally infected mice of susceptible strains and in adult mice with certain immune deficiencies, and CD8+ αβ T cells are regarded as the main effectors in anti-tumor immunity. Here we report that adult TCRβ knockout (KO) mice that lack αβ but have γδ T cells remain tumor-free after PyV infection, whereas TCRβ×δ KO mice that lack all T cells develop tumors. In addition, E26 mice, which lack NK and T cells, develop the tumors earlier than TCRβ×δ KO mice. These observations implicate γδ T and NK cells in the resistance to PyV-induced tumors. Cell lines established from PyV-induced tumors activate NK and γδ T cells both in culture and in vivo and express Rae-1, an NKG2D ligand. Moreover, these PyV tumor cells are killed by NK cells in vitro, and this cytotoxicity is prevented by treatment with NKG2D-blocking antibodies. Our findings demonstrate a protective role for NK and γδ T cells against naturally occurring virus-induced tumors and suggest the involvement of NKG2D-mediated mechanisms.
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spelling pubmed-28777382010-06-03 NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors Mishra, Rabinarayan Chen, Alex T. Welsh, Raymond M. Szomolanyi-Tsuda, Eva PLoS Pathog Research Article NK and γδ T cells can eliminate tumor cells in many experimental models, but their effect on the development of tumors caused by virus infections in vivo is not known. Polyomavirus (PyV) induces tumors in neonatally infected mice of susceptible strains and in adult mice with certain immune deficiencies, and CD8+ αβ T cells are regarded as the main effectors in anti-tumor immunity. Here we report that adult TCRβ knockout (KO) mice that lack αβ but have γδ T cells remain tumor-free after PyV infection, whereas TCRβ×δ KO mice that lack all T cells develop tumors. In addition, E26 mice, which lack NK and T cells, develop the tumors earlier than TCRβ×δ KO mice. These observations implicate γδ T and NK cells in the resistance to PyV-induced tumors. Cell lines established from PyV-induced tumors activate NK and γδ T cells both in culture and in vivo and express Rae-1, an NKG2D ligand. Moreover, these PyV tumor cells are killed by NK cells in vitro, and this cytotoxicity is prevented by treatment with NKG2D-blocking antibodies. Our findings demonstrate a protective role for NK and γδ T cells against naturally occurring virus-induced tumors and suggest the involvement of NKG2D-mediated mechanisms. Public Library of Science 2010-05-27 /pmc/articles/PMC2877738/ /pubmed/20523894 http://dx.doi.org/10.1371/journal.ppat.1000924 Text en Mishra et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mishra, Rabinarayan
Chen, Alex T.
Welsh, Raymond M.
Szomolanyi-Tsuda, Eva
NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors
title NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors
title_full NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors
title_fullStr NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors
title_full_unstemmed NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors
title_short NK Cells and γδ T Cells Mediate Resistance to Polyomavirus–Induced Tumors
title_sort nk cells and γδ t cells mediate resistance to polyomavirus–induced tumors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877738/
https://www.ncbi.nlm.nih.gov/pubmed/20523894
http://dx.doi.org/10.1371/journal.ppat.1000924
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