Cargando…

The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity

Many vector-borne pathogens evade clearance via rapid variation in their immunogenic surface expressed proteins. This is exemplified by Anaplasma marginale, a tick-borne bacterial pathogen that generates major surface protein 2 (Msp2) variants to provide for immune escape and allow long-term pathoge...

Descripción completa

Detalles Bibliográficos
Autores principales: Noh, Susan M., Zhuang, Yan, Futse, James E., Brown, Wendy C., Brayton, Kelly A., Palmer, Guy H.
Formato: Texto
Lenguaje:English
Publicado: Elsevier Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877794/
https://www.ncbi.nlm.nih.gov/pubmed/20199762
http://dx.doi.org/10.1016/j.vaccine.2010.02.067
_version_ 1782181819217608704
author Noh, Susan M.
Zhuang, Yan
Futse, James E.
Brown, Wendy C.
Brayton, Kelly A.
Palmer, Guy H.
author_facet Noh, Susan M.
Zhuang, Yan
Futse, James E.
Brown, Wendy C.
Brayton, Kelly A.
Palmer, Guy H.
author_sort Noh, Susan M.
collection PubMed
description Many vector-borne pathogens evade clearance via rapid variation in their immunogenic surface expressed proteins. This is exemplified by Anaplasma marginale, a tick-borne bacterial pathogen that generates major surface protein 2 (Msp2) variants to provide for immune escape and allow long-term pathogen persistence. In contrast to persistence following infection, immunization with a surface protein complex, which includes Msp2, induces a response that prevents infection upon challenge. We hypothesized that the immune response induced by immunization altered the anti-Msp2 antibody repertoire as compared to that induced during infection, shifting the immune response toward conserved and thus broadly protective epitopes. The antibody response to the conserved (CR) and hypervariable (HVR) regions encoded by the full set of msp2 variant alleles was determined for immunized animals prior to challenge and non-immunized, infected animals. While both groups of animals had a similar antibody repertoire in terms of breath and magnitude, the titers to the Msp2 CR were strongly correlated (p < 0.005) with control of bacteremia only in the infected animals. Among the immunized animals, there was no correlation between the breadth or magnitude of the anti-Msp2 antibody response and either complete protection from infection or control of bacteremia. This is consistent with separate immunologic mechanisms being responsible for control of bacteremia in infected animals as compared to immunized animals and suggests that conserved outer membrane proteins other than Msp2 are responsible for the complete clearance observed following challenge of vaccinees.
format Text
id pubmed-2877794
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Elsevier Science
record_format MEDLINE/PubMed
spelling pubmed-28777942010-06-10 The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity Noh, Susan M. Zhuang, Yan Futse, James E. Brown, Wendy C. Brayton, Kelly A. Palmer, Guy H. Vaccine Article Many vector-borne pathogens evade clearance via rapid variation in their immunogenic surface expressed proteins. This is exemplified by Anaplasma marginale, a tick-borne bacterial pathogen that generates major surface protein 2 (Msp2) variants to provide for immune escape and allow long-term pathogen persistence. In contrast to persistence following infection, immunization with a surface protein complex, which includes Msp2, induces a response that prevents infection upon challenge. We hypothesized that the immune response induced by immunization altered the anti-Msp2 antibody repertoire as compared to that induced during infection, shifting the immune response toward conserved and thus broadly protective epitopes. The antibody response to the conserved (CR) and hypervariable (HVR) regions encoded by the full set of msp2 variant alleles was determined for immunized animals prior to challenge and non-immunized, infected animals. While both groups of animals had a similar antibody repertoire in terms of breath and magnitude, the titers to the Msp2 CR were strongly correlated (p < 0.005) with control of bacteremia only in the infected animals. Among the immunized animals, there was no correlation between the breadth or magnitude of the anti-Msp2 antibody response and either complete protection from infection or control of bacteremia. This is consistent with separate immunologic mechanisms being responsible for control of bacteremia in infected animals as compared to immunized animals and suggests that conserved outer membrane proteins other than Msp2 are responsible for the complete clearance observed following challenge of vaccinees. Elsevier Science 2010-05-07 /pmc/articles/PMC2877794/ /pubmed/20199762 http://dx.doi.org/10.1016/j.vaccine.2010.02.067 Text en https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Noh, Susan M.
Zhuang, Yan
Futse, James E.
Brown, Wendy C.
Brayton, Kelly A.
Palmer, Guy H.
The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
title The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
title_full The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
title_fullStr The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
title_full_unstemmed The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
title_short The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
title_sort immunization-induced antibody response to the anaplasma marginale major surface protein 2 and its association with protective immunity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877794/
https://www.ncbi.nlm.nih.gov/pubmed/20199762
http://dx.doi.org/10.1016/j.vaccine.2010.02.067
work_keys_str_mv AT nohsusanm theimmunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT zhuangyan theimmunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT futsejamese theimmunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT brownwendyc theimmunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT braytonkellya theimmunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT palmerguyh theimmunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT nohsusanm immunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT zhuangyan immunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT futsejamese immunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT brownwendyc immunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT braytonkellya immunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity
AT palmerguyh immunizationinducedantibodyresponsetotheanaplasmamarginalemajorsurfaceprotein2anditsassociationwithprotectiveimmunity