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NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response

NIP/DuoxA, originally cloned as a protein capable of binding to the cell fate determinant Numb in Drosophila, was recently identified as a modulator of reactive oxygen species (ROS) production in mammalian systems. Despite biochemical and cellular studies that link NIP/DuoxA to the generation of ROS...

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Autores principales: Xie, Xiaojun, Hu, Jack, Liu, Xiping, Qin, Hanjuan, Percival-Smith, Anthony, Rao, Yong, Li, Shawn S.C.
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878171/
https://www.ncbi.nlm.nih.gov/pubmed/20567495
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author Xie, Xiaojun
Hu, Jack
Liu, Xiping
Qin, Hanjuan
Percival-Smith, Anthony
Rao, Yong
Li, Shawn S.C.
author_facet Xie, Xiaojun
Hu, Jack
Liu, Xiping
Qin, Hanjuan
Percival-Smith, Anthony
Rao, Yong
Li, Shawn S.C.
author_sort Xie, Xiaojun
collection PubMed
description NIP/DuoxA, originally cloned as a protein capable of binding to the cell fate determinant Numb in Drosophila, was recently identified as a modulator of reactive oxygen species (ROS) production in mammalian systems. Despite biochemical and cellular studies that link NIP/DuoxA to the generation of ROS through the dual oxidase (Duox) enzyme, the in vivo function of NIP/DuoxA has not been characterized to date. Here we report a genetic and functional characterization of nip in Drosophila melanogaster. We show that nip is essential for Drosophila development as nip null mutants die at the 1(st) larval instar. Expression of UAS-nip, but not UAS-Duox, rescued the lethality. To understand the function of nip beyond the early larval stage, we generated GAL4 inducible UAS-RNAi transgenes. da(G32)-GAL4 driven, ubiquitous RNAi-mediated silencing of nip led to profound abnormality in pre-adult development, crinkled wing and markedly reduced lifespan at 29°C. Compared to wild type flies, da-GAL4 induced nip-RNAi transgenic flies exhibited significantly reduced ability to survive under oxidative stress and displayed impaired mitochondrial aconitase function. Our work provides in vivo evidence for a critical role for nip in the development and oxidative stress response in Drosophila.
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spelling pubmed-28781712010-06-21 NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response Xie, Xiaojun Hu, Jack Liu, Xiping Qin, Hanjuan Percival-Smith, Anthony Rao, Yong Li, Shawn S.C. Int J Biol Sci Research Paper NIP/DuoxA, originally cloned as a protein capable of binding to the cell fate determinant Numb in Drosophila, was recently identified as a modulator of reactive oxygen species (ROS) production in mammalian systems. Despite biochemical and cellular studies that link NIP/DuoxA to the generation of ROS through the dual oxidase (Duox) enzyme, the in vivo function of NIP/DuoxA has not been characterized to date. Here we report a genetic and functional characterization of nip in Drosophila melanogaster. We show that nip is essential for Drosophila development as nip null mutants die at the 1(st) larval instar. Expression of UAS-nip, but not UAS-Duox, rescued the lethality. To understand the function of nip beyond the early larval stage, we generated GAL4 inducible UAS-RNAi transgenes. da(G32)-GAL4 driven, ubiquitous RNAi-mediated silencing of nip led to profound abnormality in pre-adult development, crinkled wing and markedly reduced lifespan at 29°C. Compared to wild type flies, da-GAL4 induced nip-RNAi transgenic flies exhibited significantly reduced ability to survive under oxidative stress and displayed impaired mitochondrial aconitase function. Our work provides in vivo evidence for a critical role for nip in the development and oxidative stress response in Drosophila. Ivyspring International Publisher 2010-05-11 /pmc/articles/PMC2878171/ /pubmed/20567495 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Xie, Xiaojun
Hu, Jack
Liu, Xiping
Qin, Hanjuan
Percival-Smith, Anthony
Rao, Yong
Li, Shawn S.C.
NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response
title NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response
title_full NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response
title_fullStr NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response
title_full_unstemmed NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response
title_short NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response
title_sort nip/duoxa is essential for drosophila embryonic development and regulates oxidative stress response
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878171/
https://www.ncbi.nlm.nih.gov/pubmed/20567495
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