Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients

We developed a real-time reverse transcription–-PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients w...

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Autores principales: Wölfel, Roman, Paweska, Janusz T., Petersen, Nadine, Grobbelaar, Antoinette A., Leman, Patricia A., Hewson, Roger, Georges-Courbot, Marie-Claude, Papa, Anna, Günther, Stephan, Drosten, Christian
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2007
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878241/
https://www.ncbi.nlm.nih.gov/pubmed/18214191
http://dx.doi.org/10.3201/eid1307.070068
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author Wölfel, Roman
Paweska, Janusz T.
Petersen, Nadine
Grobbelaar, Antoinette A.
Leman, Patricia A.
Hewson, Roger
Georges-Courbot, Marie-Claude
Papa, Anna
Günther, Stephan
Drosten, Christian
author_facet Wölfel, Roman
Paweska, Janusz T.
Petersen, Nadine
Grobbelaar, Antoinette A.
Leman, Patricia A.
Hewson, Roger
Georges-Courbot, Marie-Claude
Papa, Anna
Günther, Stephan
Drosten, Christian
author_sort Wölfel, Roman
collection PubMed
description We developed a real-time reverse transcription–-PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection.
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spelling pubmed-28782412010-06-03 Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients Wölfel, Roman Paweska, Janusz T. Petersen, Nadine Grobbelaar, Antoinette A. Leman, Patricia A. Hewson, Roger Georges-Courbot, Marie-Claude Papa, Anna Günther, Stephan Drosten, Christian Emerg Infect Dis Dispatch We developed a real-time reverse transcription–-PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection. Centers for Disease Control and Prevention 2007-07 /pmc/articles/PMC2878241/ /pubmed/18214191 http://dx.doi.org/10.3201/eid1307.070068 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Wölfel, Roman
Paweska, Janusz T.
Petersen, Nadine
Grobbelaar, Antoinette A.
Leman, Patricia A.
Hewson, Roger
Georges-Courbot, Marie-Claude
Papa, Anna
Günther, Stephan
Drosten, Christian
Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
title Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
title_full Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
title_fullStr Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
title_full_unstemmed Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
title_short Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
title_sort virus detection and monitoring of viral load in crimean-congo hemorrhagic fever virus patients
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878241/
https://www.ncbi.nlm.nih.gov/pubmed/18214191
http://dx.doi.org/10.3201/eid1307.070068
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