Targeted gene deletions in C. elegans using transposon excision

We have developed a method, MosDel, to generate targeted knock-outs of genes in C. elegans. We make use of the Mos1 transposase to excise a Mos1 transposon adjacent to the region to be deleted. The double-strand break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA...

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Detalles Bibliográficos
Autores principales: Frøkjær-Jensen, Christian, Davis, M. Wayne, Hollopeter, Gunther, Taylor, Jon, Harris, Todd, Nix, Paola, Lofgren, Rachel, Prestgard-Duke, Michael, Bastiani, Michael, Moerman, Donald G., Jorgensen, Erik M.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878396/
https://www.ncbi.nlm.nih.gov/pubmed/20418868
http://dx.doi.org/10.1038/nmeth.1454
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author Frøkjær-Jensen, Christian
Davis, M. Wayne
Hollopeter, Gunther
Taylor, Jon
Harris, Todd
Nix, Paola
Lofgren, Rachel
Prestgard-Duke, Michael
Bastiani, Michael
Moerman, Donald G.
Jorgensen, Erik M.
author_facet Frøkjær-Jensen, Christian
Davis, M. Wayne
Hollopeter, Gunther
Taylor, Jon
Harris, Todd
Nix, Paola
Lofgren, Rachel
Prestgard-Duke, Michael
Bastiani, Michael
Moerman, Donald G.
Jorgensen, Erik M.
author_sort Frøkjær-Jensen, Christian
collection PubMed
description We have developed a method, MosDel, to generate targeted knock-outs of genes in C. elegans. We make use of the Mos1 transposase to excise a Mos1 transposon adjacent to the region to be deleted. The double-strand break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.
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spelling pubmed-28783962010-12-01 Targeted gene deletions in C. elegans using transposon excision Frøkjær-Jensen, Christian Davis, M. Wayne Hollopeter, Gunther Taylor, Jon Harris, Todd Nix, Paola Lofgren, Rachel Prestgard-Duke, Michael Bastiani, Michael Moerman, Donald G. Jorgensen, Erik M. Nat Methods Article We have developed a method, MosDel, to generate targeted knock-outs of genes in C. elegans. We make use of the Mos1 transposase to excise a Mos1 transposon adjacent to the region to be deleted. The double-strand break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker. 2010-04-25 2010-06 /pmc/articles/PMC2878396/ /pubmed/20418868 http://dx.doi.org/10.1038/nmeth.1454 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Frøkjær-Jensen, Christian
Davis, M. Wayne
Hollopeter, Gunther
Taylor, Jon
Harris, Todd
Nix, Paola
Lofgren, Rachel
Prestgard-Duke, Michael
Bastiani, Michael
Moerman, Donald G.
Jorgensen, Erik M.
Targeted gene deletions in C. elegans using transposon excision
title Targeted gene deletions in C. elegans using transposon excision
title_full Targeted gene deletions in C. elegans using transposon excision
title_fullStr Targeted gene deletions in C. elegans using transposon excision
title_full_unstemmed Targeted gene deletions in C. elegans using transposon excision
title_short Targeted gene deletions in C. elegans using transposon excision
title_sort targeted gene deletions in c. elegans using transposon excision
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878396/
https://www.ncbi.nlm.nih.gov/pubmed/20418868
http://dx.doi.org/10.1038/nmeth.1454
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