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Characterization of the Ebola virus nucleoprotein–RNA complex
When Ebola virus nucleoprotein (NP) is expressed in mammalian cells, it assembles into helical structures. Here, the recombinant NP helix purified from cells expressing NP was characterized biochemically and morphologically. We found that the recombinant NP helix is associated with non-viral RNA, wh...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Society for General Microbiology
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878588/ https://www.ncbi.nlm.nih.gov/pubmed/20164259 http://dx.doi.org/10.1099/vir.0.019794-0 |
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author | Noda, Takeshi Hagiwara, Kyoji Sagara, Hiroshi Kawaoka, Yoshihiro |
author_facet | Noda, Takeshi Hagiwara, Kyoji Sagara, Hiroshi Kawaoka, Yoshihiro |
author_sort | Noda, Takeshi |
collection | PubMed |
description | When Ebola virus nucleoprotein (NP) is expressed in mammalian cells, it assembles into helical structures. Here, the recombinant NP helix purified from cells expressing NP was characterized biochemically and morphologically. We found that the recombinant NP helix is associated with non-viral RNA, which is not protected from RNase digestion and that the morphology of the helix changes depending on the environmental salt concentration. The N-terminal 450 aa residues of NP are sufficient for these properties. However, digestion of the NP-associated RNA eliminates the plasticity of the helix, suggesting that this RNA is an essential structural component of the helix, binding to individual NP molecules via the N-terminal 450 aa. These findings enhance our knowledge of Ebola virus assembly and understanding of the Ebola virus life cycle. |
format | Text |
id | pubmed-2878588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Society for General Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28785882011-06-01 Characterization of the Ebola virus nucleoprotein–RNA complex Noda, Takeshi Hagiwara, Kyoji Sagara, Hiroshi Kawaoka, Yoshihiro J Gen Virol Animal When Ebola virus nucleoprotein (NP) is expressed in mammalian cells, it assembles into helical structures. Here, the recombinant NP helix purified from cells expressing NP was characterized biochemically and morphologically. We found that the recombinant NP helix is associated with non-viral RNA, which is not protected from RNase digestion and that the morphology of the helix changes depending on the environmental salt concentration. The N-terminal 450 aa residues of NP are sufficient for these properties. However, digestion of the NP-associated RNA eliminates the plasticity of the helix, suggesting that this RNA is an essential structural component of the helix, binding to individual NP molecules via the N-terminal 450 aa. These findings enhance our knowledge of Ebola virus assembly and understanding of the Ebola virus life cycle. Society for General Microbiology 2010-06 /pmc/articles/PMC2878588/ /pubmed/20164259 http://dx.doi.org/10.1099/vir.0.019794-0 Text en Copyright © 2010, SGM |
spellingShingle | Animal Noda, Takeshi Hagiwara, Kyoji Sagara, Hiroshi Kawaoka, Yoshihiro Characterization of the Ebola virus nucleoprotein–RNA complex |
title | Characterization of the Ebola virus nucleoprotein–RNA complex |
title_full | Characterization of the Ebola virus nucleoprotein–RNA complex |
title_fullStr | Characterization of the Ebola virus nucleoprotein–RNA complex |
title_full_unstemmed | Characterization of the Ebola virus nucleoprotein–RNA complex |
title_short | Characterization of the Ebola virus nucleoprotein–RNA complex |
title_sort | characterization of the ebola virus nucleoprotein–rna complex |
topic | Animal |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878588/ https://www.ncbi.nlm.nih.gov/pubmed/20164259 http://dx.doi.org/10.1099/vir.0.019794-0 |
work_keys_str_mv | AT nodatakeshi characterizationoftheebolavirusnucleoproteinrnacomplex AT hagiwarakyoji characterizationoftheebolavirusnucleoproteinrnacomplex AT sagarahiroshi characterizationoftheebolavirusnucleoproteinrnacomplex AT kawaokayoshihiro characterizationoftheebolavirusnucleoproteinrnacomplex |