Cargando…
Cell-Free Expression of Protein Kinase A for Rapid Activity Assays
Functional protein analysis often calls for lengthy, laborious in vivo protein expression and purification, and can be complicated by the lack of stability of the purified protein. In this study, we demonstrate the feasibility of a simplified procedure for functional protein analysis on magnetic par...
Autores principales: | Leippe, Donna M., Zhao, Kate Qin, Hsiao, Kevin, Slater, Michael R. |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879223/ https://www.ncbi.nlm.nih.gov/pubmed/20520741 |
Ejemplares similares
-
A Convenient, Rapid, Sensitive, and Reliable Spectrophotometric Assay for Adenylate Kinase Activity
por: Song, Kai, et al.
Publicado: (2019) -
Microfluidic Cell Deformability Assay for Rapid and Efficient Kinase Screening with the CRISPR‐Cas9 System
por: Han, Xin, et al.
Publicado: (2016) -
Characterization of Phosphorylation Status and Kinase Activity of Src Family Kinases Expressed in Cell-Based and Cell-Free Protein Expression Systems
por: Kinoshita-Kikuta, Emiko, et al.
Publicado: (2021) -
Label-Free Assay of Protein Kinase A Activity and Inhibition Using a Peptide-Based Electrochemical Sensor
por: Cho, Hyunju, et al.
Publicado: (2021) -
An ultrasensitive fiveplex activity assay for cellular kinases
por: Smolko, Christian M., et al.
Publicado: (2019)