Direct detection of DNA methylation during single-molecule, real-time sequencing

We describe the direct detection of DNA methylation, without bisulfite conversion, through single-molecule real-time (SMRT) sequencing. In SMRT sequencing, DNA polymerases catalyze the incorporation of fluorescently labeled nucleotides into complementary nucleic acid strands. The arrival times and d...

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Detalles Bibliográficos
Autores principales: Flusberg, Benjamin A., Webster, Dale, Lee, Jessa, Travers, Kevin, Olivares, Eric, Clark, Tyson A., Korlach, Jonas, Turner, Stephen W.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879396/
https://www.ncbi.nlm.nih.gov/pubmed/20453866
http://dx.doi.org/10.1038/nmeth.1459

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879396/
https://www.ncbi.nlm.nih.gov/pubmed/20453866
http://dx.doi.org/10.1038/nmeth.1459

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