Cargando…

Direct detection of DNA methylation during single-molecule, real-time sequencing

We describe the direct detection of DNA methylation, without bisulfite conversion, through single-molecule real-time (SMRT) sequencing. In SMRT sequencing, DNA polymerases catalyze the incorporation of fluorescently labeled nucleotides into complementary nucleic acid strands. The arrival times and d...

Descripción completa

Detalles Bibliográficos
Autores principales:	Flusberg, Benjamin A., Webster, Dale, Lee, Jessa, Travers, Kevin, Olivares, Eric, Clark, Tyson A., Korlach, Jonas, Turner, Stephen W.
Formato:	Texto
Lenguaje:	English
Publicado:	2010
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879396/ https://www.ncbi.nlm.nih.gov/pubmed/20453866 http://dx.doi.org/10.1038/nmeth.1459

Ejemplares similares

Direct Detection and Sequencing of Damaged DNA Bases
por: Clark, Tyson A, et al.
Publicado: (2011)

Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing
por: Clark, Tyson A., et al.
Publicado: (2012)

Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation
por: Clark, Tyson A, et al.
Publicado: (2013)

Real-time tRNA transit on single translating ribosomes at codon resolution
por: Uemura, Sotaro, et al.
Publicado: (2010)

Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription
por: Vilfan, Igor D, et al.
Publicado: (2013)

DNA Methylation Assessed by SMRT Sequencing Is Linked to Mutations in Neisseria meningitidis Isolates
por: Sater, Mohamad R. Abdul, et al.
Publicado: (2015)

Detecting DNA Modifications from SMRT Sequencing Data by Modeling Sequence Context Dependence of Polymerase Kinetic
por: Feng, Zhixing, et al.
Publicado: (2013)

Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing
por: Genest, Paul-Andre, et al.
Publicado: (2015)

Structural and functional diversity among Type III restriction-modification systems that confer host DNA protection via methylation of the N4 atom of cytosine
por: Murray, Iain A., et al.
Publicado: (2021)

Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
por: Larkin, Joseph, et al.
Publicado: (2014)

Quantitative and multiplexed DNA methylation analysis using long-read single-molecule real-time bisulfite sequencing (SMRT-BS)
por: Yang, Yao, et al.
Publicado: (2015)

Resolving the Complexity of Human Skin Metagenomes Using Single-Molecule Sequencing
por: Tsai, Yu-Chih, et al.
Publicado: (2016)

Modeling kinetic rate variation in third generation DNA sequencing data to detect putative modifications to DNA bases
por: Schadt, Eric E., et al.
Publicado: (2013)

Length-Independent DNA Packing into Nanopore Zero-Mode Waveguides for Low-Input DNA Sequencing
por: Larkin, Joseph, et al.
Publicado: (2017)

Long, Processive Enzymatic Dna Synthesis Using 100% Dye-Labeled Terminal Phosphate-Linked Nucleotides
por: Korlach, Jonas, et al.
Publicado: (2008)

The methylomes of six bacteria
por: Murray, Iain A., et al.
Publicado: (2014)

The methylomes of six bacteria
por: Murray, Iain A., et al.
Publicado: (2012)

Complete Genome Sequence of Moraxella catarrhalis Strain CCRI-195ME, Isolated from the Middle Ear
por: Tan, Aimee, et al.
Publicado: (2017)

Genome-wide mapping of nucleosome positioning and DNA methylation within individual DNA molecules
por: Kelly, Theresa K., et al.
Publicado: (2012)

Comprehensive Methylome Characterization of Mycoplasma genitalium and Mycoplasma pneumoniae at Single-Base Resolution
por: Lluch-Senar, Maria, et al.
Publicado: (2013)

Genome-wide detection of cytosine methylation by single molecule real-time sequencing
por: Tse, O. Y. Olivia, et al.
Publicado: (2021)

Genome-Wide Methylation Patterns in Salmonella enterica Subsp. enterica Serovars
por: Pirone-Davies, Cary, et al.
Publicado: (2015)

Characterization of fusion genes and the significantly expressed fusion isoforms in breast cancer by hybrid sequencing
por: Weirather, Jason L., et al.
Publicado: (2015)

Direct detection of methylation in genomic DNA
por: Bart, A., et al.
Publicado: (2005)

Genetic Stabilization of the Drug-Resistant PMEN1 Pneumococcus Lineage by Its Distinctive DpnIII Restriction-Modification System
por: Eutsey, Rory A., et al.
Publicado: (2015)

Complete Genome Sequences of Two Escherichia coli O145:H28 Outbreak Strains of Food Origin
por: Cooper, Kerry K., et al.
Publicado: (2014)

RNA-directed DNA Methylation
por: Erdmann, Robert M., et al.
Publicado: (2020)

The role of DNA methylation in directing the functional organization of the cancer epigenome
por: Lay, Fides D., et al.
Publicado: (2015)

Single-molecule methylation profiles of cell-free DNA in cancer with nanopore sequencing
por: Lau, Billy T., et al.
Publicado: (2023)

Returning to more finished genomes
por: Korlach, Jonas
Publicado: (2014)

Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA
por: Statham, Aaron L., et al.
Publicado: (2012)

DNA Damage, Homology-Directed Repair, and DNA Methylation
por: Cuozzo, Concetta, et al.
Publicado: (2007)

Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules
por: Schwartz, Jerrod J, et al.
Publicado: (2012)

A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
por: Fabian, Roberto, et al.
Publicado: (2021)

Identification of Restriction-Modification Systems of Bifidobacterium animalis subsp. lactis CNCM I-2494 by SMRT Sequencing and Associated Methylome Analysis
por: O′Connell Motherway, Mary, et al.
Publicado: (2014)

Integration of sperm ncRNA-directed DNA methylation and DNA methylation-directed histone retention in epigenetic transgenerational inheritance
por: Beck, Daniel, et al.
Publicado: (2021)

DNA binding fluorescent proteins for the direct visualization of large DNA molecules
por: Lee, Seonghyun, et al.
Publicado: (2016)

Comparative genomics of enterohemorrhagic Escherichia coli O145:H28 demonstrates a common evolutionary lineage with Escherichia coli O157:H7
por: Cooper, Kerry K, et al.
Publicado: (2014)

Real-time single-molecule observation of rolling-circle DNA replication
por: Tanner, Nathan A., et al.
Publicado: (2009)

Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping
por: Gabrieli, Tslil, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_hmr4stm4baaqj0cng77r98tlud