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Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA

In trypanosomes a 39 nucleotide exon, the spliced leader (SL) is donated to all mRNAs from a small RNA, the SL RNA, by trans-splicing. Since the discovery of trans-splicing in trypanosomes two decades ago, numerous attempts failed to reconstitute the reaction in vitro. In this study, a crude whole-c...

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Detalles Bibliográficos
Autores principales:	Shaked, Hadassa, Wachtel, Chaim, Tulinski, Pawel, Yahia, Nasreen Hag, Barda, Omer, Darzynkiewicz, Edward, Nilsen, Timothy W., Michaeli, Shulamit
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879531/ https://www.ncbi.nlm.nih.gov/pubmed/20159996 http://dx.doi.org/10.1093/nar/gkq065

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