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Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease
Porphyromonas gingivalis and Treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis that P. gingivalis and T. denticola are synergistic in terms of virulence; using a model of mixed microb...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879544/ https://www.ncbi.nlm.nih.gov/pubmed/20592756 http://dx.doi.org/10.1155/2010/605125 |
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author | Verma, Raj K. Rajapakse, Sunethra Meka, Archana Hamrick, Clayton Pola, Sheela Bhattacharyya, Indraneel Nair, Madhu Wallet, Shannon M. Aukhil, Ikramuddin Kesavalu, Lakshmyya |
author_facet | Verma, Raj K. Rajapakse, Sunethra Meka, Archana Hamrick, Clayton Pola, Sheela Bhattacharyya, Indraneel Nair, Madhu Wallet, Shannon M. Aukhil, Ikramuddin Kesavalu, Lakshmyya |
author_sort | Verma, Raj K. |
collection | PubMed |
description | Porphyromonas gingivalis and Treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis that P. gingivalis and T. denticola are synergistic in terms of virulence; using a model of mixed microbial infection in rats. Groups of rats were orally infected with either P. gingivalis or T. denticola or mixed microbial infections for 7 and 12 weeks. P. gingivalis genomic DNA was detected more frequently by PCR than T. denticola. Both bacteria induced significantly high IgG, IgG2b, IgG1, IgG2a antibody levels indicating a stimulation of Th1 and Th2 immune response. Radiographic and morphometric measurements demonstrated that rats infected with the mixed infection exhibited significantly more alveolar bone loss than shaminfected control rats. Histology revealed apical migration of junctional epithelium, rete ridge elongation, and crestal alveolar bone resorption; resembling periodontal disease lesion. These results showed that P. gingivalis and T. denticola exhibit no synergistic virulence in a rat model of periodontal disease. |
format | Text |
id | pubmed-2879544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-28795442010-06-30 Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease Verma, Raj K. Rajapakse, Sunethra Meka, Archana Hamrick, Clayton Pola, Sheela Bhattacharyya, Indraneel Nair, Madhu Wallet, Shannon M. Aukhil, Ikramuddin Kesavalu, Lakshmyya Interdiscip Perspect Infect Dis Research Article Porphyromonas gingivalis and Treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis that P. gingivalis and T. denticola are synergistic in terms of virulence; using a model of mixed microbial infection in rats. Groups of rats were orally infected with either P. gingivalis or T. denticola or mixed microbial infections for 7 and 12 weeks. P. gingivalis genomic DNA was detected more frequently by PCR than T. denticola. Both bacteria induced significantly high IgG, IgG2b, IgG1, IgG2a antibody levels indicating a stimulation of Th1 and Th2 immune response. Radiographic and morphometric measurements demonstrated that rats infected with the mixed infection exhibited significantly more alveolar bone loss than shaminfected control rats. Histology revealed apical migration of junctional epithelium, rete ridge elongation, and crestal alveolar bone resorption; resembling periodontal disease lesion. These results showed that P. gingivalis and T. denticola exhibit no synergistic virulence in a rat model of periodontal disease. Hindawi Publishing Corporation 2010 2010-05-31 /pmc/articles/PMC2879544/ /pubmed/20592756 http://dx.doi.org/10.1155/2010/605125 Text en Copyright © 2010 Raj K. Verma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Verma, Raj K. Rajapakse, Sunethra Meka, Archana Hamrick, Clayton Pola, Sheela Bhattacharyya, Indraneel Nair, Madhu Wallet, Shannon M. Aukhil, Ikramuddin Kesavalu, Lakshmyya Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
title |
Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
title_full |
Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
title_fullStr |
Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
title_full_unstemmed |
Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
title_short |
Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
title_sort | porphyromonas gingivalis and treponema denticola mixed microbial infection in a rat model of periodontal disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879544/ https://www.ncbi.nlm.nih.gov/pubmed/20592756 http://dx.doi.org/10.1155/2010/605125 |
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