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Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients
BACKGROUND: T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leuke...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2880023/ https://www.ncbi.nlm.nih.gov/pubmed/20470401 http://dx.doi.org/10.1186/1479-5876-8-47 |
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author | Li, Yangqiu Geng, Suxia Yin, Qingsong Chen, Shaohua Yang, Lijian Wu, Xiuli Li, Bo Du, Xin Schmidt, Christian A Przybylski, Grzegorz K |
author_facet | Li, Yangqiu Geng, Suxia Yin, Qingsong Chen, Shaohua Yang, Lijian Wu, Xiuli Li, Bo Du, Xin Schmidt, Christian A Przybylski, Grzegorz K |
author_sort | Li, Yangqiu |
collection | PubMed |
description | BACKGROUND: T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients. METHODS: Quantitative analysis of δRec-ψJα signal joint T cell receptor excision circles (sjTRECs) was performed in PBMCs, CD3+, CD4+ and CD8+T cells by real-time PCR, and the analysis of 23 TRBV-D1 sjTRECs was performed by semi-nested PCR. Forty eight CML cases in chronic phase (CML-CP) were selected for this study and 17 healthy individuals served as controls. RESULTS: The levels of δRec-ψJα sjTRECs in PBMCs, CD3+, CD4+, and CD8+ T cells were significantly decreased in CML patients, compared with control groups. Moreover, the numbers of detectable TRBV subfamily sjTRECs, as well as the frequency of particular TRBV-BD1 sjTRECs in patients with CML were significantly lower than those from healthy individuals. CONCLUSIONS: We observed decreased levels of recent thymic emigrants in CD4+ and CD8+ T cells that may underlay the persistent immunodeficiency in CML patients. |
format | Text |
id | pubmed-2880023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28800232010-06-03 Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients Li, Yangqiu Geng, Suxia Yin, Qingsong Chen, Shaohua Yang, Lijian Wu, Xiuli Li, Bo Du, Xin Schmidt, Christian A Przybylski, Grzegorz K J Transl Med Research BACKGROUND: T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients. METHODS: Quantitative analysis of δRec-ψJα signal joint T cell receptor excision circles (sjTRECs) was performed in PBMCs, CD3+, CD4+ and CD8+T cells by real-time PCR, and the analysis of 23 TRBV-D1 sjTRECs was performed by semi-nested PCR. Forty eight CML cases in chronic phase (CML-CP) were selected for this study and 17 healthy individuals served as controls. RESULTS: The levels of δRec-ψJα sjTRECs in PBMCs, CD3+, CD4+, and CD8+ T cells were significantly decreased in CML patients, compared with control groups. Moreover, the numbers of detectable TRBV subfamily sjTRECs, as well as the frequency of particular TRBV-BD1 sjTRECs in patients with CML were significantly lower than those from healthy individuals. CONCLUSIONS: We observed decreased levels of recent thymic emigrants in CD4+ and CD8+ T cells that may underlay the persistent immunodeficiency in CML patients. BioMed Central 2010-05-14 /pmc/articles/PMC2880023/ /pubmed/20470401 http://dx.doi.org/10.1186/1479-5876-8-47 Text en Copyright ©2010 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Li, Yangqiu Geng, Suxia Yin, Qingsong Chen, Shaohua Yang, Lijian Wu, Xiuli Li, Bo Du, Xin Schmidt, Christian A Przybylski, Grzegorz K Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients |
title | Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients |
title_full | Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients |
title_fullStr | Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients |
title_full_unstemmed | Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients |
title_short | Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients |
title_sort | decreased level of recent thymic emigrants in cd4+ and cd8+t cells from cml patients |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2880023/ https://www.ncbi.nlm.nih.gov/pubmed/20470401 http://dx.doi.org/10.1186/1479-5876-8-47 |
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