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Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities

BACKGROUND: The term endothelial progenitor cells (EPCs) is currently used to refer to cell populations which are quite dissimilar in terms of biological properties. This study provides a detailed molecular fingerprint for two EPC subtypes: early EPCs (eEPCs) and outgrowth endothelial cells (OECs)....

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Autores principales: Medina, Reinhold J, O'Neill, Christina L, Sweeney, Mark, Guduric-Fuchs, Jasenka, Gardiner, Tom A, Simpson, David A, Stitt, Alan W
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881111/
https://www.ncbi.nlm.nih.gov/pubmed/20465783
http://dx.doi.org/10.1186/1755-8794-3-18
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author Medina, Reinhold J
O'Neill, Christina L
Sweeney, Mark
Guduric-Fuchs, Jasenka
Gardiner, Tom A
Simpson, David A
Stitt, Alan W
author_facet Medina, Reinhold J
O'Neill, Christina L
Sweeney, Mark
Guduric-Fuchs, Jasenka
Gardiner, Tom A
Simpson, David A
Stitt, Alan W
author_sort Medina, Reinhold J
collection PubMed
description BACKGROUND: The term endothelial progenitor cells (EPCs) is currently used to refer to cell populations which are quite dissimilar in terms of biological properties. This study provides a detailed molecular fingerprint for two EPC subtypes: early EPCs (eEPCs) and outgrowth endothelial cells (OECs). METHODS: Human blood-derived eEPCs and OECs were characterised by using genome-wide transcriptional profiling, 2D protein electrophoresis, and electron microscopy. Comparative analysis at the transcript and protein level included monocytes and mature endothelial cells as reference cell types. RESULTS: Our data show that eEPCs and OECs have strikingly different gene expression signatures. Many highly expressed transcripts in eEPCs are haematopoietic specific (RUNX1, WAS, LYN) with links to immunity and inflammation (TLRs, CD14, HLAs), whereas many transcripts involved in vascular development and angiogenesis-related signalling pathways (Tie2, eNOS, Ephrins) are highly expressed in OECs. Comparative analysis with monocytes and mature endothelial cells clusters eEPCs with monocytes, while OECs segment with endothelial cells. Similarly, proteomic analysis revealed that 90% of spots identified by 2-D gel analysis are common between OECs and endothelial cells while eEPCs share 77% with monocytes. In line with the expression pattern of caveolins and cadherins identified by microarray analysis, ultrastructural evaluation highlighted the presence of caveolae and adherens junctions only in OECs. CONCLUSIONS: This study provides evidence that eEPCs are haematopoietic cells with a molecular phenotype linked to monocytes; whereas OECs exhibit commitment to the endothelial lineage. These findings indicate that OECs might be an attractive cell candidate for inducing therapeutic angiogenesis, while eEPC should be used with caution because of their monocytic nature.
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spelling pubmed-28811112010-06-05 Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities Medina, Reinhold J O'Neill, Christina L Sweeney, Mark Guduric-Fuchs, Jasenka Gardiner, Tom A Simpson, David A Stitt, Alan W BMC Med Genomics Research article BACKGROUND: The term endothelial progenitor cells (EPCs) is currently used to refer to cell populations which are quite dissimilar in terms of biological properties. This study provides a detailed molecular fingerprint for two EPC subtypes: early EPCs (eEPCs) and outgrowth endothelial cells (OECs). METHODS: Human blood-derived eEPCs and OECs were characterised by using genome-wide transcriptional profiling, 2D protein electrophoresis, and electron microscopy. Comparative analysis at the transcript and protein level included monocytes and mature endothelial cells as reference cell types. RESULTS: Our data show that eEPCs and OECs have strikingly different gene expression signatures. Many highly expressed transcripts in eEPCs are haematopoietic specific (RUNX1, WAS, LYN) with links to immunity and inflammation (TLRs, CD14, HLAs), whereas many transcripts involved in vascular development and angiogenesis-related signalling pathways (Tie2, eNOS, Ephrins) are highly expressed in OECs. Comparative analysis with monocytes and mature endothelial cells clusters eEPCs with monocytes, while OECs segment with endothelial cells. Similarly, proteomic analysis revealed that 90% of spots identified by 2-D gel analysis are common between OECs and endothelial cells while eEPCs share 77% with monocytes. In line with the expression pattern of caveolins and cadherins identified by microarray analysis, ultrastructural evaluation highlighted the presence of caveolae and adherens junctions only in OECs. CONCLUSIONS: This study provides evidence that eEPCs are haematopoietic cells with a molecular phenotype linked to monocytes; whereas OECs exhibit commitment to the endothelial lineage. These findings indicate that OECs might be an attractive cell candidate for inducing therapeutic angiogenesis, while eEPC should be used with caution because of their monocytic nature. BioMed Central 2010-05-13 /pmc/articles/PMC2881111/ /pubmed/20465783 http://dx.doi.org/10.1186/1755-8794-3-18 Text en Copyright ©2010 Medina et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Medina, Reinhold J
O'Neill, Christina L
Sweeney, Mark
Guduric-Fuchs, Jasenka
Gardiner, Tom A
Simpson, David A
Stitt, Alan W
Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities
title Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities
title_full Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities
title_fullStr Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities
title_full_unstemmed Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities
title_short Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities
title_sort molecular analysis of endothelial progenitor cell (epc) subtypes reveals two distinct cell populations with different identities
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881111/
https://www.ncbi.nlm.nih.gov/pubmed/20465783
http://dx.doi.org/10.1186/1755-8794-3-18
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