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Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin-E(2) (PGE(2)). While keratinocytes are a major PGE(2) source, epidermal melanocytes (EM) also express PGE(2)-production machinery. It is unclear whether EM-produced PGE(2) contributes to UVR-induced sk...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Blackwell Publishing Ltd
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881306/ https://www.ncbi.nlm.nih.gov/pubmed/20236442 http://dx.doi.org/10.1111/j.1755-148X.2010.00696.x |
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author | Gledhill, Karl Rhodes, Lesley E Brownrigg, Margaret Haylett, Ann K Masoodi, Mojgan Thody, Anthony J Nicolaou, Anna Tobin, Desmond J |
author_facet | Gledhill, Karl Rhodes, Lesley E Brownrigg, Margaret Haylett, Ann K Masoodi, Mojgan Thody, Anthony J Nicolaou, Anna Tobin, Desmond J |
author_sort | Gledhill, Karl |
collection | PubMed |
description | Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin-E(2) (PGE(2)). While keratinocytes are a major PGE(2) source, epidermal melanocytes (EM) also express PGE(2)-production machinery. It is unclear whether EM-produced PGE(2) contributes to UVR-induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype-1 and -4 donors, followed by assessment of PGE(2) production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase-A(2), cyclooxygenase-1, cytoplasmic prostaglandin-E synthase and microsomal prostaglandin-E synthase-1, -2. Epidermal melanocytes produced PGE(2) under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase-2 (COX-2) mRNA and a selective COX-2 inhibitor (NS-398) reduced PGE(2) production. Ultraviolet B-induced PGE(2) production was positively correlated with skin phototype-1, despite variability between individual EM donors. By contrast, there was no correlation between PGE(2) production by EM and their melanogenic status. Thus, EM may contribute to UVR-induced erythema, with role of donor skin phototype more important than their melanogenic status. |
format | Text |
id | pubmed-2881306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-28813062010-06-09 Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner Gledhill, Karl Rhodes, Lesley E Brownrigg, Margaret Haylett, Ann K Masoodi, Mojgan Thody, Anthony J Nicolaou, Anna Tobin, Desmond J Pigment Cell Melanoma Res Original Articles Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin-E(2) (PGE(2)). While keratinocytes are a major PGE(2) source, epidermal melanocytes (EM) also express PGE(2)-production machinery. It is unclear whether EM-produced PGE(2) contributes to UVR-induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype-1 and -4 donors, followed by assessment of PGE(2) production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase-A(2), cyclooxygenase-1, cytoplasmic prostaglandin-E synthase and microsomal prostaglandin-E synthase-1, -2. Epidermal melanocytes produced PGE(2) under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase-2 (COX-2) mRNA and a selective COX-2 inhibitor (NS-398) reduced PGE(2) production. Ultraviolet B-induced PGE(2) production was positively correlated with skin phototype-1, despite variability between individual EM donors. By contrast, there was no correlation between PGE(2) production by EM and their melanogenic status. Thus, EM may contribute to UVR-induced erythema, with role of donor skin phototype more important than their melanogenic status. Blackwell Publishing Ltd 2010-06 2010-03-17 /pmc/articles/PMC2881306/ /pubmed/20236442 http://dx.doi.org/10.1111/j.1755-148X.2010.00696.x Text en © 2010 Blackwell Munksgaard http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Original Articles Gledhill, Karl Rhodes, Lesley E Brownrigg, Margaret Haylett, Ann K Masoodi, Mojgan Thody, Anthony J Nicolaou, Anna Tobin, Desmond J Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner |
title | Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner |
title_full | Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner |
title_fullStr | Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner |
title_full_unstemmed | Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner |
title_short | Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner |
title_sort | prostaglandin-e(2) is produced by adult human epidermal melanocytes in response to uvb in a melanogenesis-independent manner |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881306/ https://www.ncbi.nlm.nih.gov/pubmed/20236442 http://dx.doi.org/10.1111/j.1755-148X.2010.00696.x |
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