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Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner

Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin-E(2) (PGE(2)). While keratinocytes are a major PGE(2) source, epidermal melanocytes (EM) also express PGE(2)-production machinery. It is unclear whether EM-produced PGE(2) contributes to UVR-induced sk...

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Detalles Bibliográficos
Autores principales: Gledhill, Karl, Rhodes, Lesley E, Brownrigg, Margaret, Haylett, Ann K, Masoodi, Mojgan, Thody, Anthony J, Nicolaou, Anna, Tobin, Desmond J
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881306/
https://www.ncbi.nlm.nih.gov/pubmed/20236442
http://dx.doi.org/10.1111/j.1755-148X.2010.00696.x
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author Gledhill, Karl
Rhodes, Lesley E
Brownrigg, Margaret
Haylett, Ann K
Masoodi, Mojgan
Thody, Anthony J
Nicolaou, Anna
Tobin, Desmond J
author_facet Gledhill, Karl
Rhodes, Lesley E
Brownrigg, Margaret
Haylett, Ann K
Masoodi, Mojgan
Thody, Anthony J
Nicolaou, Anna
Tobin, Desmond J
author_sort Gledhill, Karl
collection PubMed
description Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin-E(2) (PGE(2)). While keratinocytes are a major PGE(2) source, epidermal melanocytes (EM) also express PGE(2)-production machinery. It is unclear whether EM-produced PGE(2) contributes to UVR-induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype-1 and -4 donors, followed by assessment of PGE(2) production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase-A(2), cyclooxygenase-1, cytoplasmic prostaglandin-E synthase and microsomal prostaglandin-E synthase-1, -2. Epidermal melanocytes produced PGE(2) under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase-2 (COX-2) mRNA and a selective COX-2 inhibitor (NS-398) reduced PGE(2) production. Ultraviolet B-induced PGE(2) production was positively correlated with skin phototype-1, despite variability between individual EM donors. By contrast, there was no correlation between PGE(2) production by EM and their melanogenic status. Thus, EM may contribute to UVR-induced erythema, with role of donor skin phototype more important than their melanogenic status.
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spelling pubmed-28813062010-06-09 Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner Gledhill, Karl Rhodes, Lesley E Brownrigg, Margaret Haylett, Ann K Masoodi, Mojgan Thody, Anthony J Nicolaou, Anna Tobin, Desmond J Pigment Cell Melanoma Res Original Articles Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin-E(2) (PGE(2)). While keratinocytes are a major PGE(2) source, epidermal melanocytes (EM) also express PGE(2)-production machinery. It is unclear whether EM-produced PGE(2) contributes to UVR-induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype-1 and -4 donors, followed by assessment of PGE(2) production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase-A(2), cyclooxygenase-1, cytoplasmic prostaglandin-E synthase and microsomal prostaglandin-E synthase-1, -2. Epidermal melanocytes produced PGE(2) under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase-2 (COX-2) mRNA and a selective COX-2 inhibitor (NS-398) reduced PGE(2) production. Ultraviolet B-induced PGE(2) production was positively correlated with skin phototype-1, despite variability between individual EM donors. By contrast, there was no correlation between PGE(2) production by EM and their melanogenic status. Thus, EM may contribute to UVR-induced erythema, with role of donor skin phototype more important than their melanogenic status. Blackwell Publishing Ltd 2010-06 2010-03-17 /pmc/articles/PMC2881306/ /pubmed/20236442 http://dx.doi.org/10.1111/j.1755-148X.2010.00696.x Text en © 2010 Blackwell Munksgaard http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Articles
Gledhill, Karl
Rhodes, Lesley E
Brownrigg, Margaret
Haylett, Ann K
Masoodi, Mojgan
Thody, Anthony J
Nicolaou, Anna
Tobin, Desmond J
Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
title Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
title_full Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
title_fullStr Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
title_full_unstemmed Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
title_short Prostaglandin-E(2) is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
title_sort prostaglandin-e(2) is produced by adult human epidermal melanocytes in response to uvb in a melanogenesis-independent manner
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881306/
https://www.ncbi.nlm.nih.gov/pubmed/20236442
http://dx.doi.org/10.1111/j.1755-148X.2010.00696.x
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