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Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis
Mastitis, the inflammation of the mammary gland, is an important disease affecting dairy animals worldwide. The disease is caused by mammary pathogenic bacteria and Escherichia coli are frequently implicated. Virulence factors of mammary pathogenic E. coli are only partially known and intramammary c...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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EDP Sciences
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881416/ https://www.ncbi.nlm.nih.gov/pubmed/20416261 http://dx.doi.org/10.1051/vetres/2010025 |
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author | Elazar, Sharon Gonen, Erez Livneh-Kol, Ayala Rosenshine, Ilan Shpigel, Nahum Y. |
author_facet | Elazar, Sharon Gonen, Erez Livneh-Kol, Ayala Rosenshine, Ilan Shpigel, Nahum Y. |
author_sort | Elazar, Sharon |
collection | PubMed |
description | Mastitis, the inflammation of the mammary gland, is an important disease affecting dairy animals worldwide. The disease is caused by mammary pathogenic bacteria and Escherichia coli are frequently implicated. Virulence factors of mammary pathogenic E. coli are only partially known and intramammary challenge with LPS elicits neutrophil recruitment in experimental bovine and murine mastitis models. We have previously shown that neutrophil recruitment in LPS-induced murine mastitis is strictly dependent on mammary alveolar macrophages. However, the relative role of alveolar macrophages and blood neutrophils in E. coli mastitis is not well defined. To this end, we selectively depleted mammary alveolar macrophages or blood neutrophils before intramammary challenge with E. coli strain P4 (ECP4). Mice depleted of alveolar macrophages prior to intramammary challenge recruited neutrophils normally and restricted bacterial growth and interstitial invasion. Importantly however, upon depletion of alveolar macrophages, ECP4 invaded the mammary alveolar epithelial cells and formed intracellular bacterial communities. In contrast, neutrophil depletion prior to intramammary infection with ECP4 was associated with unrestricted bacterial growth, tissue damage, severe sepsis and mortality. This study suggests that neutrophils but not alveolar macrophages provide essential antimicrobial defense against mammary pathogenic E. coli. Furthermore, we show here similar invasion after depletion of alveolar macrophages as in our previous studies showing that LPS/TLR4 signaling on alveolar macrophages abrogates ECP4 invasion of the mammary epithelium. Interestingly, similar ECP4 invasion and formation of intracellular communities were also observed following intramammary infection of either iNOS gene-deficient or IL-1 receptor type 1 gene-deficient mice. |
format | Text |
id | pubmed-2881416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-28814162011-07-01 Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis Elazar, Sharon Gonen, Erez Livneh-Kol, Ayala Rosenshine, Ilan Shpigel, Nahum Y. Vet Res Original Article Mastitis, the inflammation of the mammary gland, is an important disease affecting dairy animals worldwide. The disease is caused by mammary pathogenic bacteria and Escherichia coli are frequently implicated. Virulence factors of mammary pathogenic E. coli are only partially known and intramammary challenge with LPS elicits neutrophil recruitment in experimental bovine and murine mastitis models. We have previously shown that neutrophil recruitment in LPS-induced murine mastitis is strictly dependent on mammary alveolar macrophages. However, the relative role of alveolar macrophages and blood neutrophils in E. coli mastitis is not well defined. To this end, we selectively depleted mammary alveolar macrophages or blood neutrophils before intramammary challenge with E. coli strain P4 (ECP4). Mice depleted of alveolar macrophages prior to intramammary challenge recruited neutrophils normally and restricted bacterial growth and interstitial invasion. Importantly however, upon depletion of alveolar macrophages, ECP4 invaded the mammary alveolar epithelial cells and formed intracellular bacterial communities. In contrast, neutrophil depletion prior to intramammary infection with ECP4 was associated with unrestricted bacterial growth, tissue damage, severe sepsis and mortality. This study suggests that neutrophils but not alveolar macrophages provide essential antimicrobial defense against mammary pathogenic E. coli. Furthermore, we show here similar invasion after depletion of alveolar macrophages as in our previous studies showing that LPS/TLR4 signaling on alveolar macrophages abrogates ECP4 invasion of the mammary epithelium. Interestingly, similar ECP4 invasion and formation of intracellular communities were also observed following intramammary infection of either iNOS gene-deficient or IL-1 receptor type 1 gene-deficient mice. EDP Sciences 2010-04-26 2010 /pmc/articles/PMC2881416/ /pubmed/20416261 http://dx.doi.org/10.1051/vetres/2010025 Text en © INRA, EDP Sciences, 2010 |
spellingShingle | Original Article Elazar, Sharon Gonen, Erez Livneh-Kol, Ayala Rosenshine, Ilan Shpigel, Nahum Y. Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis |
title | Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis |
title_full | Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis |
title_fullStr | Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis |
title_full_unstemmed | Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis |
title_short | Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis |
title_sort | essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute escherichia coli mastitis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881416/ https://www.ncbi.nlm.nih.gov/pubmed/20416261 http://dx.doi.org/10.1051/vetres/2010025 |
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