A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

BACKGROUND: The high-throughput anchoring of genetic markers into contigs is required for many ongoing physical mapping projects. Multidimentional BAC pooling strategies for PCR-based screening of large insert libraries is a widely used alternative to high density filter hybridisation of bacterial c...

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Detalles Bibliográficos
Autores principales: Vu, Giang TH, Caligari, Peter DS, Wilkinson, Mike J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881884/
https://www.ncbi.nlm.nih.gov/pubmed/20462427
http://dx.doi.org/10.1186/1471-2164-11-301

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881884/
https://www.ncbi.nlm.nih.gov/pubmed/20462427
http://dx.doi.org/10.1186/1471-2164-11-301

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