Cargando…

Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness

BACKGROUND: Influenza A, including avian influenza, is a major public health threat in developed and developing countries. Rapid and accurate detection is a key component of strategies to contain spread of infection, and the efficient diagnosis of influenza-like-illness is essential to protect healt...

Descripción completa

Detalles Bibliográficos
Autores principales:	Szewczuk, Elektra, Thapa, Kiran, Anninos, Terry, McPhie, Kenneth, Higgins, Geoff, Dwyer, Dominic E, Stanley, Keith K, Iredell, Jonathan R
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Technical Advance
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881921/ https://www.ncbi.nlm.nih.gov/pubmed/20459845 http://dx.doi.org/10.1186/1471-2334-10-113

Ejemplares similares

Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection
por: Stanley, Keith K., et al.
Publicado: (2005)

Sequential multiplex PCR assay for determining capsular serotypes of colonizing S. pneumoniae
por: Jourdain, Sarah, et al.
Publicado: (2011)

Development and validation of a multiplex-PCR assay for X-linked intellectual disability
por: Jorge, Paula, et al.
Publicado: (2013)

A tissue biopsy-based epigenetic multiplex PCR assay for prostate cancer detection
por: Van Neste, Leander, et al.
Publicado: (2012)

Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape
por: Snellenberg, Suzanne, et al.
Publicado: (2012)

Multiplexed-tandem PCR (MT-PCR) assay to detect and differentiate gastrointestinal nematodes of alpacas
por: Rashid, Mohammed H., et al.
Publicado: (2018)

Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms
por: You, Huey-Ling, et al.
Publicado: (2017)

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients
por: Drago, Lorenzo, et al.
Publicado: (2004)

Detection of Norovirus genogroup I and II by multiplex real-time RT- PCR using a 3'-minor groove binder-DNA probe
por: Hoehne, Marina, et al.
Publicado: (2006)

Simultaneous detection of lung fusions using a multiplex RT-PCR next generation sequencing-based approach: a multi-institutional research study
por: Vaughn, Cecily P., et al.
Publicado: (2018)

Targeted capture-based NGS is superior to multiplex PCR-based NGS for hereditary BRCA1 and BRCA2 gene analysis in FFPE tumor samples
por: Zakrzewski, Falk, et al.
Publicado: (2019)

A reliable method for the detection of BRCA1 and BRCA2 mutations in fixed tumour tissue utilising multiplex PCR-based targeted next generation sequencing
por: Ellison, Gillian, et al.
Publicado: (2015)

Detection of group a streptococcal pharyngitis by quantitative PCR
por: Dunne, Eileen M, et al.
Publicado: (2013)

Quantitative threefold allele-specific PCR (QuanTAS-PCR) for highly sensitive JAK2 V617F mutant allele detection
por: Zapparoli, Giada V, et al.
Publicado: (2013)

Improvement of In Situ PCR by Optimization of PCR Cycle Number and Proteinase K Concentration: Localization of X Chromosome-Linked Phosphoglycerate Kinase-1 Gene in Mouse Reproductive Organs
por: Hishikawa, Yoshitaka, et al.
Publicado: (2009)

Detection of large deletions in the LDL receptor gene with quantitative PCR methods
por: Damgaard, Dorte, et al.
Publicado: (2005)

Evaluation of a novel real-time PCR test based on the ssrA gene for the identification of group B streptococci in vaginal swabs
por: Wernecke, Martina, et al.
Publicado: (2009)

Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR)
por: Turner, Andrew, et al.
Publicado: (2016)

Development of a Multiplex Tandem PCR (MT-PCR) Assay for the Detection of Emerging SARS-CoV-2 Variants
por: Hale, Richard, et al.
Publicado: (2021)

Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid
por: Khot, Prasanna D, et al.
Publicado: (2008)

PCR amplification of a triple-repeat genetic target directly from whole blood in 15 minutes as a proof-of-principle PCR study for direct sample analysis for a clinically relevant target
por: Connelly, Christopher M, et al.
Publicado: (2014)

Development and validation of a high throughput, closed tube method for the determination of haemoglobin alpha gene (HBA1 and HBA2) numbers by gene ratio assay copy enumeration-PCR (GRACE-PCR)
por: Turner, Andrew, et al.
Publicado: (2015)

Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction
por: Pandori, Mark W, et al.
Publicado: (2006)

Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
por: Lorente, Aiala, et al.
Publicado: (2008)

Fast detection of Noroviruses using a real-time PCR assay and automated sample preparation
por: Schmid, Michael, et al.
Publicado: (2004)

Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR
por: Grimholt, Runa M, et al.
Publicado: (2014)

Rapid Detection of Chlamydia trachomatis and Typing of the Lymphogranuloma venereum associated L-Serovars by TaqMan PCR
por: Schaeffer, Anke, et al.
Publicado: (2008)

COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma
por: Pritchard, Colin C, et al.
Publicado: (2010)

Development and evaluation of a real-time one step Reverse-Transcriptase PCR for quantitation of Chandipura Virus
por: Kumar, Satyendra, et al.
Publicado: (2008)

An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis
por: Fabri, Loraine V., et al.
Publicado: (2021)

Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva
por: Orrù, Germano, et al.
Publicado: (2006)

SNPs and real-time quantitative PCR method for constitutional allelic copy number determination, the VPREB1 marker case
por: Frigerio, Marcello, et al.
Publicado: (2011)

Invasive anisakiasis by the parasite Anisakis pegreffii (Nematoda: Anisakidae): diagnosis by real-time PCR hydrolysis probe system and immunoblotting assay
por: Mattiucci, Simonetta, et al.
Publicado: (2017)

Sample-to-Answer and Routine Real-Time RT-PCR: A Comparison of Different Platforms for SARS-CoV-2 Detection
por: Wen, Donghua, et al.
Publicado: (2021)

Touchdown General Primer (GP5+/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus
por: Evans, Mark F, et al.
Publicado: (2005)

Conversion of a molecular classifier obtained by gene expression profiling into a classifier based on real-time PCR: a prognosis predictor for gliomas
por: Kawarazaki, Satoru, et al.
Publicado: (2010)

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler(® )Real-time PCR
por: Kabamba-Mukadi, Benoît, et al.
Publicado: (2005)

Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples
por: Beissner, Marcus, et al.
Publicado: (2019)

Evaluation of PCR procedures for detecting and quantifying Leishmania donovani DNA in large numbers of dried human blood samples from a visceral leishmaniasis focus in Northern Ethiopia
por: Abbasi, Ibrahim, et al.
Publicado: (2013)

Development and Validation of Two RT-qPCR Diagnostic Assays for Detecting Severe Acute Respiratory Syndrome Coronavirus 2 Genomic Targets across Two Specimen Types
por: Miller, Eric W., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_noe05njnke971fo5q38ling2cc