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A novel prokaryotic vector for identification and selection of recombinants: Direct use of the vector for expression studies in E. coli
BACKGROUND: The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have pro...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2882348/ https://www.ncbi.nlm.nih.gov/pubmed/20459760 http://dx.doi.org/10.1186/1475-2859-9-30 |