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Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus

An alignment of upstream regions of anaerobically induced genes in Staphylococcus aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in Streptomyces coelicolor. Gel shift experiments of selected upstream regions demonstrated that the redox-sensing regulator Rex of...

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Autores principales: Pagels, Martin, Fuchs, Stephan, Pané-Farré, Jan, Kohler, Christian, Menschner, Leonhard, Hecker, Michael, McNamarra, Peter J, Bauer, Mikael C, von Wachenfeldt, Claes, Liebeke, Manuel, Lalk, Michael, Sander, Gunnar, von Eiff, Christof, Proctor, Richard A, Engelmann, Susanne
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883068/
https://www.ncbi.nlm.nih.gov/pubmed/20374494
http://dx.doi.org/10.1111/j.1365-2958.2010.07105.x
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author Pagels, Martin
Fuchs, Stephan
Pané-Farré, Jan
Kohler, Christian
Menschner, Leonhard
Hecker, Michael
McNamarra, Peter J
Bauer, Mikael C
von Wachenfeldt, Claes
Liebeke, Manuel
Lalk, Michael
Sander, Gunnar
von Eiff, Christof
Proctor, Richard A
Engelmann, Susanne
author_facet Pagels, Martin
Fuchs, Stephan
Pané-Farré, Jan
Kohler, Christian
Menschner, Leonhard
Hecker, Michael
McNamarra, Peter J
Bauer, Mikael C
von Wachenfeldt, Claes
Liebeke, Manuel
Lalk, Michael
Sander, Gunnar
von Eiff, Christof
Proctor, Richard A
Engelmann, Susanne
author_sort Pagels, Martin
collection PubMed
description An alignment of upstream regions of anaerobically induced genes in Staphylococcus aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in Streptomyces coelicolor. Gel shift experiments of selected upstream regions demonstrated that the redox-sensing regulator Rex of S. aureus binds to this inverted repeat. The binding sequence – TTGTGAAW(4)TTCACAA – is highly conserved in S. aureus. Rex binding to this sequence leads to the repression of genes located downstream. The binding activity of Rex is enhanced by NAD(+) while NADH, which competes with NAD(+) for Rex binding, decreases the activity of Rex. The impact of Rex on global protein synthesis and on the activity of fermentation pathways under aerobic and anaerobic conditions was analysed by using a rex-deficient strain. A direct regulatory effect of Rex on the expression of pathways that lead to anaerobic NAD(+) regeneration, such as lactate, formate and ethanol formation, nitrate respiration, and ATP synthesis, is verified. Rex can be considered a central regulator of anaerobic metabolism in S. aureus. Since the activity of lactate dehydrogenase enables S. aureus to resist NO stress and thus the innate immune response, our data suggest that deactivation of Rex is a prerequisite for this phenomenon.
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spelling pubmed-28830682010-06-15 Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus Pagels, Martin Fuchs, Stephan Pané-Farré, Jan Kohler, Christian Menschner, Leonhard Hecker, Michael McNamarra, Peter J Bauer, Mikael C von Wachenfeldt, Claes Liebeke, Manuel Lalk, Michael Sander, Gunnar von Eiff, Christof Proctor, Richard A Engelmann, Susanne Mol Microbiol Research Articles An alignment of upstream regions of anaerobically induced genes in Staphylococcus aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in Streptomyces coelicolor. Gel shift experiments of selected upstream regions demonstrated that the redox-sensing regulator Rex of S. aureus binds to this inverted repeat. The binding sequence – TTGTGAAW(4)TTCACAA – is highly conserved in S. aureus. Rex binding to this sequence leads to the repression of genes located downstream. The binding activity of Rex is enhanced by NAD(+) while NADH, which competes with NAD(+) for Rex binding, decreases the activity of Rex. The impact of Rex on global protein synthesis and on the activity of fermentation pathways under aerobic and anaerobic conditions was analysed by using a rex-deficient strain. A direct regulatory effect of Rex on the expression of pathways that lead to anaerobic NAD(+) regeneration, such as lactate, formate and ethanol formation, nitrate respiration, and ATP synthesis, is verified. Rex can be considered a central regulator of anaerobic metabolism in S. aureus. Since the activity of lactate dehydrogenase enables S. aureus to resist NO stress and thus the innate immune response, our data suggest that deactivation of Rex is a prerequisite for this phenomenon. Blackwell Publishing Ltd 2010-06 2010-03-30 /pmc/articles/PMC2883068/ /pubmed/20374494 http://dx.doi.org/10.1111/j.1365-2958.2010.07105.x Text en © 2010 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Pagels, Martin
Fuchs, Stephan
Pané-Farré, Jan
Kohler, Christian
Menschner, Leonhard
Hecker, Michael
McNamarra, Peter J
Bauer, Mikael C
von Wachenfeldt, Claes
Liebeke, Manuel
Lalk, Michael
Sander, Gunnar
von Eiff, Christof
Proctor, Richard A
Engelmann, Susanne
Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
title Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
title_full Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
title_fullStr Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
title_full_unstemmed Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
title_short Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
title_sort redox sensing by a rex-family repressor is involved in the regulation of anaerobic gene expression in staphylococcus aureus
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883068/
https://www.ncbi.nlm.nih.gov/pubmed/20374494
http://dx.doi.org/10.1111/j.1365-2958.2010.07105.x
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