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CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY

The silent information regulator (Sir2) family proteins are NAD(+)-dependent deacetylases. Although a few substrates have been identified, functions of the bacteria Sir2-like protein (CobB) still remain unclear. Here the role of CobB on Escherichia coli chemotaxis was investigated. We used Western b...

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Autores principales: Li, Ru, Gu, Jing, Chen, Yuan-Yuan, Xiao, Chuan-Le, Wang, Li-Wei, Zhang, Zhi-Ping, Bi, Li-Jun, Wei, Hong-Ping, Wang, Xu-De, Deng, Jiao-Yu, Zhang, Xian-En
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883070/
https://www.ncbi.nlm.nih.gov/pubmed/20345663
http://dx.doi.org/10.1111/j.1365-2958.2010.07125.x
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author Li, Ru
Gu, Jing
Chen, Yuan-Yuan
Xiao, Chuan-Le
Wang, Li-Wei
Zhang, Zhi-Ping
Bi, Li-Jun
Wei, Hong-Ping
Wang, Xu-De
Deng, Jiao-Yu
Zhang, Xian-En
author_facet Li, Ru
Gu, Jing
Chen, Yuan-Yuan
Xiao, Chuan-Le
Wang, Li-Wei
Zhang, Zhi-Ping
Bi, Li-Jun
Wei, Hong-Ping
Wang, Xu-De
Deng, Jiao-Yu
Zhang, Xian-En
author_sort Li, Ru
collection PubMed
description The silent information regulator (Sir2) family proteins are NAD(+)-dependent deacetylases. Although a few substrates have been identified, functions of the bacteria Sir2-like protein (CobB) still remain unclear. Here the role of CobB on Escherichia coli chemotaxis was investigated. We used Western blotting and mass spectrometry to show that the response regulator CheY is a substrate of CobB. Surface plasmon resonance (SPR) indicated that acetylation affects the interaction between CheY and the flagellar switch protein FliM. The presence of intact flagella in knockout strains ΔcobB, Δacs, Δ(cobB) Δ(acs), Δ(cheA) Δ(cheZ), Δ(cheA) Δ(cheZ) Δ(cobB) and Δ(cheA) Δ(cheZ) Δ(acs) was confirmed by electron microscopy. Genetic analysis of these knockout strains showed that: (i) the ΔcobB mutant exhibited reduced responses to chemotactic stimuli in chemotactic assays, whereas the Δacs mutant was indistinguishable from the parental strain, (ii) CheY from the ΔcobB mutant showed a higher level of acetylation, indicating that CobB can mediate the deacetylation of CheY in vivo, and (iii) deletion of cobB reversed the phenotype of Δ(cheA) Δ(cheZ). Our findings suggest that CobB regulates E. coli chemotaxis by deacetylating CheY. Thus a new function of bacterial cobB was identified and also new insights of regulation of bacterial chemotaxis were provided.
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spelling pubmed-28830702010-06-15 CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY Li, Ru Gu, Jing Chen, Yuan-Yuan Xiao, Chuan-Le Wang, Li-Wei Zhang, Zhi-Ping Bi, Li-Jun Wei, Hong-Ping Wang, Xu-De Deng, Jiao-Yu Zhang, Xian-En Mol Microbiol Research Articles The silent information regulator (Sir2) family proteins are NAD(+)-dependent deacetylases. Although a few substrates have been identified, functions of the bacteria Sir2-like protein (CobB) still remain unclear. Here the role of CobB on Escherichia coli chemotaxis was investigated. We used Western blotting and mass spectrometry to show that the response regulator CheY is a substrate of CobB. Surface plasmon resonance (SPR) indicated that acetylation affects the interaction between CheY and the flagellar switch protein FliM. The presence of intact flagella in knockout strains ΔcobB, Δacs, Δ(cobB) Δ(acs), Δ(cheA) Δ(cheZ), Δ(cheA) Δ(cheZ) Δ(cobB) and Δ(cheA) Δ(cheZ) Δ(acs) was confirmed by electron microscopy. Genetic analysis of these knockout strains showed that: (i) the ΔcobB mutant exhibited reduced responses to chemotactic stimuli in chemotactic assays, whereas the Δacs mutant was indistinguishable from the parental strain, (ii) CheY from the ΔcobB mutant showed a higher level of acetylation, indicating that CobB can mediate the deacetylation of CheY in vivo, and (iii) deletion of cobB reversed the phenotype of Δ(cheA) Δ(cheZ). Our findings suggest that CobB regulates E. coli chemotaxis by deacetylating CheY. Thus a new function of bacterial cobB was identified and also new insights of regulation of bacterial chemotaxis were provided. Blackwell Publishing Ltd 2010-06 2010-04-13 /pmc/articles/PMC2883070/ /pubmed/20345663 http://dx.doi.org/10.1111/j.1365-2958.2010.07125.x Text en © 2010 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Li, Ru
Gu, Jing
Chen, Yuan-Yuan
Xiao, Chuan-Le
Wang, Li-Wei
Zhang, Zhi-Ping
Bi, Li-Jun
Wei, Hong-Ping
Wang, Xu-De
Deng, Jiao-Yu
Zhang, Xian-En
CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY
title CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY
title_full CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY
title_fullStr CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY
title_full_unstemmed CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY
title_short CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY
title_sort cobb regulates escherichia coli chemotaxis by deacetylating the response regulator chey
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883070/
https://www.ncbi.nlm.nih.gov/pubmed/20345663
http://dx.doi.org/10.1111/j.1365-2958.2010.07125.x
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