Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures

After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report review...

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Detalles Bibliográficos
Autores principales: Nagayama, Kuniaki, Danev, Radostin
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883085/
https://www.ncbi.nlm.nih.gov/pubmed/20585379
http://dx.doi.org/10.1007/s12551-008-0006-z
Descripción
Sumario:After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report reviews the state of the art for phase plates and what is innovated with them in biological electron microscopy. The extension of thin-film phase plates to the material-less type using electrostatic field or magnetic field is also addressed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12551-008-0006-z) contains supplementary material, which is available to authorized users.

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