Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures

After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report review...

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Detalles Bibliográficos
Autores principales: Nagayama, Kuniaki, Danev, Radostin
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883085/
https://www.ncbi.nlm.nih.gov/pubmed/20585379
http://dx.doi.org/10.1007/s12551-008-0006-z
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author Nagayama, Kuniaki
Danev, Radostin
author_facet Nagayama, Kuniaki
Danev, Radostin
author_sort Nagayama, Kuniaki
collection PubMed
description After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report reviews the state of the art for phase plates and what is innovated with them in biological electron microscopy. The extension of thin-film phase plates to the material-less type using electrostatic field or magnetic field is also addressed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12551-008-0006-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-28830852010-06-21 Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures Nagayama, Kuniaki Danev, Radostin Biophys Rev Review After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report reviews the state of the art for phase plates and what is innovated with them in biological electron microscopy. The extension of thin-film phase plates to the material-less type using electrostatic field or magnetic field is also addressed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12551-008-0006-z) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-01-17 /pmc/articles/PMC2883085/ /pubmed/20585379 http://dx.doi.org/10.1007/s12551-008-0006-z Text en © The Author(s) 2008 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Review
Nagayama, Kuniaki
Danev, Radostin
Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures
title Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures
title_full Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures
title_fullStr Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures
title_full_unstemmed Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures
title_short Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures
title_sort phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883085/
https://www.ncbi.nlm.nih.gov/pubmed/20585379
http://dx.doi.org/10.1007/s12551-008-0006-z
work_keys_str_mv AT nagayamakuniaki phaseplateelectronmicroscopyanovelimagingtooltorevealclosetolifenanostructures
AT danevradostin phaseplateelectronmicroscopyanovelimagingtooltorevealclosetolifenanostructures

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