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The Scottish Structural Proteomics Facility: targets, methods and outputs
The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report ref...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer Netherlands
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883930/ https://www.ncbi.nlm.nih.gov/pubmed/20419351 http://dx.doi.org/10.1007/s10969-010-9090-y |
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author | Oke, Muse Carter, Lester G. Johnson, Kenneth A. Liu, Huanting McMahon, Stephen A. Yan, Xuan Kerou, Melina Weikart, Nadine D. Kadi, Nadia Sheikh, Md. Arif Schmelz, Stefan Dorward, Mark Zawadzki, Michal Cozens, Christopher Falconer, Helen Powers, Helen Overton, Ian M. van Niekerk, C. A. Johannes Peng, Xu Patel, Prakash Garrett, Roger A. Prangishvili, David Botting, Catherine H. Coote, Peter J. Dryden, David T. F. Barton, Geoffrey J. Schwarz-Linek, Ulrich Challis, Gregory L. Taylor, Garry L. White, Malcolm F. Naismith, James H. |
author_facet | Oke, Muse Carter, Lester G. Johnson, Kenneth A. Liu, Huanting McMahon, Stephen A. Yan, Xuan Kerou, Melina Weikart, Nadine D. Kadi, Nadia Sheikh, Md. Arif Schmelz, Stefan Dorward, Mark Zawadzki, Michal Cozens, Christopher Falconer, Helen Powers, Helen Overton, Ian M. van Niekerk, C. A. Johannes Peng, Xu Patel, Prakash Garrett, Roger A. Prangishvili, David Botting, Catherine H. Coote, Peter J. Dryden, David T. F. Barton, Geoffrey J. Schwarz-Linek, Ulrich Challis, Gregory L. Taylor, Garry L. White, Malcolm F. Naismith, James H. |
author_sort | Oke, Muse |
collection | PubMed |
description | The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10969-010-9090-y) contains supplementary material, which is available to authorized users. |
format | Text |
id | pubmed-2883930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-28839302010-06-21 The Scottish Structural Proteomics Facility: targets, methods and outputs Oke, Muse Carter, Lester G. Johnson, Kenneth A. Liu, Huanting McMahon, Stephen A. Yan, Xuan Kerou, Melina Weikart, Nadine D. Kadi, Nadia Sheikh, Md. Arif Schmelz, Stefan Dorward, Mark Zawadzki, Michal Cozens, Christopher Falconer, Helen Powers, Helen Overton, Ian M. van Niekerk, C. A. Johannes Peng, Xu Patel, Prakash Garrett, Roger A. Prangishvili, David Botting, Catherine H. Coote, Peter J. Dryden, David T. F. Barton, Geoffrey J. Schwarz-Linek, Ulrich Challis, Gregory L. Taylor, Garry L. White, Malcolm F. Naismith, James H. J Struct Funct Genomics Article The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10969-010-9090-y) contains supplementary material, which is available to authorized users. Springer Netherlands 2010-04-24 2010 /pmc/articles/PMC2883930/ /pubmed/20419351 http://dx.doi.org/10.1007/s10969-010-9090-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Oke, Muse Carter, Lester G. Johnson, Kenneth A. Liu, Huanting McMahon, Stephen A. Yan, Xuan Kerou, Melina Weikart, Nadine D. Kadi, Nadia Sheikh, Md. Arif Schmelz, Stefan Dorward, Mark Zawadzki, Michal Cozens, Christopher Falconer, Helen Powers, Helen Overton, Ian M. van Niekerk, C. A. Johannes Peng, Xu Patel, Prakash Garrett, Roger A. Prangishvili, David Botting, Catherine H. Coote, Peter J. Dryden, David T. F. Barton, Geoffrey J. Schwarz-Linek, Ulrich Challis, Gregory L. Taylor, Garry L. White, Malcolm F. Naismith, James H. The Scottish Structural Proteomics Facility: targets, methods and outputs |
title | The Scottish Structural Proteomics Facility: targets, methods and outputs |
title_full | The Scottish Structural Proteomics Facility: targets, methods and outputs |
title_fullStr | The Scottish Structural Proteomics Facility: targets, methods and outputs |
title_full_unstemmed | The Scottish Structural Proteomics Facility: targets, methods and outputs |
title_short | The Scottish Structural Proteomics Facility: targets, methods and outputs |
title_sort | scottish structural proteomics facility: targets, methods and outputs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883930/ https://www.ncbi.nlm.nih.gov/pubmed/20419351 http://dx.doi.org/10.1007/s10969-010-9090-y |
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