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The Scottish Structural Proteomics Facility: targets, methods and outputs

The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report ref...

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Autores principales: Oke, Muse, Carter, Lester G., Johnson, Kenneth A., Liu, Huanting, McMahon, Stephen A., Yan, Xuan, Kerou, Melina, Weikart, Nadine D., Kadi, Nadia, Sheikh, Md. Arif, Schmelz, Stefan, Dorward, Mark, Zawadzki, Michal, Cozens, Christopher, Falconer, Helen, Powers, Helen, Overton, Ian M., van Niekerk, C. A. Johannes, Peng, Xu, Patel, Prakash, Garrett, Roger A., Prangishvili, David, Botting, Catherine H., Coote, Peter J., Dryden, David T. F., Barton, Geoffrey J., Schwarz-Linek, Ulrich, Challis, Gregory L., Taylor, Garry L., White, Malcolm F., Naismith, James H.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883930/
https://www.ncbi.nlm.nih.gov/pubmed/20419351
http://dx.doi.org/10.1007/s10969-010-9090-y
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author Oke, Muse
Carter, Lester G.
Johnson, Kenneth A.
Liu, Huanting
McMahon, Stephen A.
Yan, Xuan
Kerou, Melina
Weikart, Nadine D.
Kadi, Nadia
Sheikh, Md. Arif
Schmelz, Stefan
Dorward, Mark
Zawadzki, Michal
Cozens, Christopher
Falconer, Helen
Powers, Helen
Overton, Ian M.
van Niekerk, C. A. Johannes
Peng, Xu
Patel, Prakash
Garrett, Roger A.
Prangishvili, David
Botting, Catherine H.
Coote, Peter J.
Dryden, David T. F.
Barton, Geoffrey J.
Schwarz-Linek, Ulrich
Challis, Gregory L.
Taylor, Garry L.
White, Malcolm F.
Naismith, James H.
author_facet Oke, Muse
Carter, Lester G.
Johnson, Kenneth A.
Liu, Huanting
McMahon, Stephen A.
Yan, Xuan
Kerou, Melina
Weikart, Nadine D.
Kadi, Nadia
Sheikh, Md. Arif
Schmelz, Stefan
Dorward, Mark
Zawadzki, Michal
Cozens, Christopher
Falconer, Helen
Powers, Helen
Overton, Ian M.
van Niekerk, C. A. Johannes
Peng, Xu
Patel, Prakash
Garrett, Roger A.
Prangishvili, David
Botting, Catherine H.
Coote, Peter J.
Dryden, David T. F.
Barton, Geoffrey J.
Schwarz-Linek, Ulrich
Challis, Gregory L.
Taylor, Garry L.
White, Malcolm F.
Naismith, James H.
author_sort Oke, Muse
collection PubMed
description The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10969-010-9090-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-28839302010-06-21 The Scottish Structural Proteomics Facility: targets, methods and outputs Oke, Muse Carter, Lester G. Johnson, Kenneth A. Liu, Huanting McMahon, Stephen A. Yan, Xuan Kerou, Melina Weikart, Nadine D. Kadi, Nadia Sheikh, Md. Arif Schmelz, Stefan Dorward, Mark Zawadzki, Michal Cozens, Christopher Falconer, Helen Powers, Helen Overton, Ian M. van Niekerk, C. A. Johannes Peng, Xu Patel, Prakash Garrett, Roger A. Prangishvili, David Botting, Catherine H. Coote, Peter J. Dryden, David T. F. Barton, Geoffrey J. Schwarz-Linek, Ulrich Challis, Gregory L. Taylor, Garry L. White, Malcolm F. Naismith, James H. J Struct Funct Genomics Article The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10969-010-9090-y) contains supplementary material, which is available to authorized users. Springer Netherlands 2010-04-24 2010 /pmc/articles/PMC2883930/ /pubmed/20419351 http://dx.doi.org/10.1007/s10969-010-9090-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Oke, Muse
Carter, Lester G.
Johnson, Kenneth A.
Liu, Huanting
McMahon, Stephen A.
Yan, Xuan
Kerou, Melina
Weikart, Nadine D.
Kadi, Nadia
Sheikh, Md. Arif
Schmelz, Stefan
Dorward, Mark
Zawadzki, Michal
Cozens, Christopher
Falconer, Helen
Powers, Helen
Overton, Ian M.
van Niekerk, C. A. Johannes
Peng, Xu
Patel, Prakash
Garrett, Roger A.
Prangishvili, David
Botting, Catherine H.
Coote, Peter J.
Dryden, David T. F.
Barton, Geoffrey J.
Schwarz-Linek, Ulrich
Challis, Gregory L.
Taylor, Garry L.
White, Malcolm F.
Naismith, James H.
The Scottish Structural Proteomics Facility: targets, methods and outputs
title The Scottish Structural Proteomics Facility: targets, methods and outputs
title_full The Scottish Structural Proteomics Facility: targets, methods and outputs
title_fullStr The Scottish Structural Proteomics Facility: targets, methods and outputs
title_full_unstemmed The Scottish Structural Proteomics Facility: targets, methods and outputs
title_short The Scottish Structural Proteomics Facility: targets, methods and outputs
title_sort scottish structural proteomics facility: targets, methods and outputs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883930/
https://www.ncbi.nlm.nih.gov/pubmed/20419351
http://dx.doi.org/10.1007/s10969-010-9090-y
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