Cargando…

Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth

When conditions are unfavorable, virtually all living cells have the capability of entering a resting state termed quiescence or G0. Many aspects of the quiescence program as well as the mechanisms governing the entry and exit from quiescence remain poorly understood. Previous studies using the budd...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Lei, Sutter, Benjamin M., Ye, Xinyue, Tu, Benjamin P.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883942/
https://www.ncbi.nlm.nih.gov/pubmed/20427572
http://dx.doi.org/10.1091/mbc.E10-01-0056
_version_ 1782182290030329856
author Shi, Lei
Sutter, Benjamin M.
Ye, Xinyue
Tu, Benjamin P.
author_facet Shi, Lei
Sutter, Benjamin M.
Ye, Xinyue
Tu, Benjamin P.
author_sort Shi, Lei
collection PubMed
description When conditions are unfavorable, virtually all living cells have the capability of entering a resting state termed quiescence or G0. Many aspects of the quiescence program as well as the mechanisms governing the entry and exit from quiescence remain poorly understood. Previous studies using the budding yeast Saccharomyces cerevisiae have shown that upon entry into stationary phase, a quiescent cell population emerges that is heavier in density than nonquiescent cells. Here, we show that total intracellular trehalose and glycogen content exhibits substantial correlation with the density of individual cells both in stationary phase batch cultures and during continuous growth. During prolonged quiescence, trehalose stores are often maintained in favor over glycogen, perhaps to fulfill its numerous stress-protectant functions. Immediately upon exit from quiescence, cells preferentially metabolize trehalose over other fuel sources. Moreover, cells lacking trehalose initiate growth more slowly and frequently exhibit poor survivability. Together, our results support the view that trehalose, which is more stable than other carbohydrates, provides an enduring source of energy that helps drive cell cycle progression upon return to growth.
format Text
id pubmed-2883942
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-28839422010-08-30 Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth Shi, Lei Sutter, Benjamin M. Ye, Xinyue Tu, Benjamin P. Mol Biol Cell Articles When conditions are unfavorable, virtually all living cells have the capability of entering a resting state termed quiescence or G0. Many aspects of the quiescence program as well as the mechanisms governing the entry and exit from quiescence remain poorly understood. Previous studies using the budding yeast Saccharomyces cerevisiae have shown that upon entry into stationary phase, a quiescent cell population emerges that is heavier in density than nonquiescent cells. Here, we show that total intracellular trehalose and glycogen content exhibits substantial correlation with the density of individual cells both in stationary phase batch cultures and during continuous growth. During prolonged quiescence, trehalose stores are often maintained in favor over glycogen, perhaps to fulfill its numerous stress-protectant functions. Immediately upon exit from quiescence, cells preferentially metabolize trehalose over other fuel sources. Moreover, cells lacking trehalose initiate growth more slowly and frequently exhibit poor survivability. Together, our results support the view that trehalose, which is more stable than other carbohydrates, provides an enduring source of energy that helps drive cell cycle progression upon return to growth. The American Society for Cell Biology 2010-06-15 /pmc/articles/PMC2883942/ /pubmed/20427572 http://dx.doi.org/10.1091/mbc.E10-01-0056 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Shi, Lei
Sutter, Benjamin M.
Ye, Xinyue
Tu, Benjamin P.
Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth
title Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth
title_full Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth
title_fullStr Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth
title_full_unstemmed Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth
title_short Trehalose Is a Key Determinant of the Quiescent Metabolic State That Fuels Cell Cycle Progression upon Return to Growth
title_sort trehalose is a key determinant of the quiescent metabolic state that fuels cell cycle progression upon return to growth
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883942/
https://www.ncbi.nlm.nih.gov/pubmed/20427572
http://dx.doi.org/10.1091/mbc.E10-01-0056
work_keys_str_mv AT shilei trehaloseisakeydeterminantofthequiescentmetabolicstatethatfuelscellcycleprogressionuponreturntogrowth
AT sutterbenjaminm trehaloseisakeydeterminantofthequiescentmetabolicstatethatfuelscellcycleprogressionuponreturntogrowth
AT yexinyue trehaloseisakeydeterminantofthequiescentmetabolicstatethatfuelscellcycleprogressionuponreturntogrowth
AT tubenjaminp trehaloseisakeydeterminantofthequiescentmetabolicstatethatfuelscellcycleprogressionuponreturntogrowth