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Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression
Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we i...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883943/ https://www.ncbi.nlm.nih.gov/pubmed/20410140 http://dx.doi.org/10.1091/mbc.E09-05-0364 |
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author | Wang, Huajin Wei, Enhui Quiroga, Ariel D. Sun, Xuejin Touret, Nicolas Lehner, Richard |
author_facet | Wang, Huajin Wei, Enhui Quiroga, Ariel D. Sun, Xuejin Touret, Nicolas Lehner, Richard |
author_sort | Wang, Huajin |
collection | PubMed |
description | Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in cytosolic LD dynamics. We have found that TGH deficiency resulted in decreased size and increased number of LDs in hepatocytes. Using fluorescent fatty acid analogues to trace LD formation, we observed that TGH deficiency did not affect the formation of nascent LDs on the ER. However, the rate of lipid transfer into preformed LDs was significantly slower in the absence of TGH. Absence of TGH expression resulted in increased levels of membrane diacylglycerol and augmented phospholipid synthesis, which may be responsible for the delayed lipid transfer. Therefore, altered maturation (growth) rather than nascent formation (de novo synthesis) may be responsible for the observed morphological changes of LDs in TGH-deficient hepatocytes. |
format | Text |
id | pubmed-2883943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28839432010-08-30 Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression Wang, Huajin Wei, Enhui Quiroga, Ariel D. Sun, Xuejin Touret, Nicolas Lehner, Richard Mol Biol Cell Articles Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in cytosolic LD dynamics. We have found that TGH deficiency resulted in decreased size and increased number of LDs in hepatocytes. Using fluorescent fatty acid analogues to trace LD formation, we observed that TGH deficiency did not affect the formation of nascent LDs on the ER. However, the rate of lipid transfer into preformed LDs was significantly slower in the absence of TGH. Absence of TGH expression resulted in increased levels of membrane diacylglycerol and augmented phospholipid synthesis, which may be responsible for the delayed lipid transfer. Therefore, altered maturation (growth) rather than nascent formation (de novo synthesis) may be responsible for the observed morphological changes of LDs in TGH-deficient hepatocytes. The American Society for Cell Biology 2010-06-15 /pmc/articles/PMC2883943/ /pubmed/20410140 http://dx.doi.org/10.1091/mbc.E09-05-0364 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Wang, Huajin Wei, Enhui Quiroga, Ariel D. Sun, Xuejin Touret, Nicolas Lehner, Richard Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression |
title | Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression |
title_full | Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression |
title_fullStr | Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression |
title_full_unstemmed | Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression |
title_short | Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression |
title_sort | altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883943/ https://www.ncbi.nlm.nih.gov/pubmed/20410140 http://dx.doi.org/10.1091/mbc.E09-05-0364 |
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