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Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression

Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we i...

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Detalles Bibliográficos
Autores principales: Wang, Huajin, Wei, Enhui, Quiroga, Ariel D., Sun, Xuejin, Touret, Nicolas, Lehner, Richard
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883943/
https://www.ncbi.nlm.nih.gov/pubmed/20410140
http://dx.doi.org/10.1091/mbc.E09-05-0364
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author Wang, Huajin
Wei, Enhui
Quiroga, Ariel D.
Sun, Xuejin
Touret, Nicolas
Lehner, Richard
author_facet Wang, Huajin
Wei, Enhui
Quiroga, Ariel D.
Sun, Xuejin
Touret, Nicolas
Lehner, Richard
author_sort Wang, Huajin
collection PubMed
description Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in cytosolic LD dynamics. We have found that TGH deficiency resulted in decreased size and increased number of LDs in hepatocytes. Using fluorescent fatty acid analogues to trace LD formation, we observed that TGH deficiency did not affect the formation of nascent LDs on the ER. However, the rate of lipid transfer into preformed LDs was significantly slower in the absence of TGH. Absence of TGH expression resulted in increased levels of membrane diacylglycerol and augmented phospholipid synthesis, which may be responsible for the delayed lipid transfer. Therefore, altered maturation (growth) rather than nascent formation (de novo synthesis) may be responsible for the observed morphological changes of LDs in TGH-deficient hepatocytes.
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spelling pubmed-28839432010-08-30 Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression Wang, Huajin Wei, Enhui Quiroga, Ariel D. Sun, Xuejin Touret, Nicolas Lehner, Richard Mol Biol Cell Articles Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in cytosolic LD dynamics. We have found that TGH deficiency resulted in decreased size and increased number of LDs in hepatocytes. Using fluorescent fatty acid analogues to trace LD formation, we observed that TGH deficiency did not affect the formation of nascent LDs on the ER. However, the rate of lipid transfer into preformed LDs was significantly slower in the absence of TGH. Absence of TGH expression resulted in increased levels of membrane diacylglycerol and augmented phospholipid synthesis, which may be responsible for the delayed lipid transfer. Therefore, altered maturation (growth) rather than nascent formation (de novo synthesis) may be responsible for the observed morphological changes of LDs in TGH-deficient hepatocytes. The American Society for Cell Biology 2010-06-15 /pmc/articles/PMC2883943/ /pubmed/20410140 http://dx.doi.org/10.1091/mbc.E09-05-0364 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Wang, Huajin
Wei, Enhui
Quiroga, Ariel D.
Sun, Xuejin
Touret, Nicolas
Lehner, Richard
Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression
title Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression
title_full Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression
title_fullStr Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression
title_full_unstemmed Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression
title_short Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression
title_sort altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883943/
https://www.ncbi.nlm.nih.gov/pubmed/20410140
http://dx.doi.org/10.1091/mbc.E09-05-0364
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