DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis

In Synthetic Biology, de novo synthesis of GC-rich constructs poses a major challenge because of secondary structure formation and mispriming. While there are many web-based tools for codon optimizing difficult regions, no method currently exists that allows for potentially phenotypically important...

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Detalles Bibliográficos
Autores principales: Jensen, Michael A., Fukushima, Marilyn, Davis, Ronald W.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883997/
https://www.ncbi.nlm.nih.gov/pubmed/20552011
http://dx.doi.org/10.1371/journal.pone.0011024

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883997/
https://www.ncbi.nlm.nih.gov/pubmed/20552011
http://dx.doi.org/10.1371/journal.pone.0011024

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