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DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis
In Synthetic Biology, de novo synthesis of GC-rich constructs poses a major challenge because of secondary structure formation and mispriming. While there are many web-based tools for codon optimizing difficult regions, no method currently exists that allows for potentially phenotypically important...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883997/ https://www.ncbi.nlm.nih.gov/pubmed/20552011 http://dx.doi.org/10.1371/journal.pone.0011024 |