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CRISPR-Cas: an adaptive immunity system in prokaryotes
Most of the archaea and numerous bacteria possess an elaborate system of adaptive immunity to mobile genetic elements known as the CRISPR (clustered regularly interspaced short palindromic repeats)-associated system (CRISPR-Cas), which consists of arrays of short repeats interspersed with unique DNA...
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Formato: | Texto |
Lenguaje: | English |
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Biology Reports
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884157/ https://www.ncbi.nlm.nih.gov/pubmed/20556198 http://dx.doi.org/10.3410/B1-95 |
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author | Koonin, Eugene V Makarova, Kira S |
author_facet | Koonin, Eugene V Makarova, Kira S |
author_sort | Koonin, Eugene V |
collection | PubMed |
description | Most of the archaea and numerous bacteria possess an elaborate system of adaptive immunity to mobile genetic elements known as the CRISPR (clustered regularly interspaced short palindromic repeats)-associated system (CRISPR-Cas), which consists of arrays of short repeats interspersed with unique DNA spacers and adjacent operons encompassing CRISPR-associated (cas) genes with predicted and, in some cases, experimentally validated nuclease, helicase, and polymerase activities. The system functions by integrating fragments of alien DNA between the repeats and employing their transcripts to degrade the DNA of the respective invading elements via an RNA interference-like mechanism. The CRISPR-Cas system is a case of apparent Lamarckian inheritance. |
format | Text |
id | pubmed-2884157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Biology Reports |
record_format | MEDLINE/PubMed |
spelling | pubmed-28841572010-10-14 CRISPR-Cas: an adaptive immunity system in prokaryotes Koonin, Eugene V Makarova, Kira S F1000 Biol Rep Review Article Most of the archaea and numerous bacteria possess an elaborate system of adaptive immunity to mobile genetic elements known as the CRISPR (clustered regularly interspaced short palindromic repeats)-associated system (CRISPR-Cas), which consists of arrays of short repeats interspersed with unique DNA spacers and adjacent operons encompassing CRISPR-associated (cas) genes with predicted and, in some cases, experimentally validated nuclease, helicase, and polymerase activities. The system functions by integrating fragments of alien DNA between the repeats and employing their transcripts to degrade the DNA of the respective invading elements via an RNA interference-like mechanism. The CRISPR-Cas system is a case of apparent Lamarckian inheritance. Biology Reports 2009-12-09 /pmc/articles/PMC2884157/ /pubmed/20556198 http://dx.doi.org/10.3410/B1-95 Text en © 2009 Faculty of 1000 Ltd http://creativecommons.org/licenses/by-nc/3.0/legalcode This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use this work for commercial purposes |
spellingShingle | Review Article Koonin, Eugene V Makarova, Kira S CRISPR-Cas: an adaptive immunity system in prokaryotes |
title | CRISPR-Cas: an adaptive immunity system in prokaryotes |
title_full | CRISPR-Cas: an adaptive immunity system in prokaryotes |
title_fullStr | CRISPR-Cas: an adaptive immunity system in prokaryotes |
title_full_unstemmed | CRISPR-Cas: an adaptive immunity system in prokaryotes |
title_short | CRISPR-Cas: an adaptive immunity system in prokaryotes |
title_sort | crispr-cas: an adaptive immunity system in prokaryotes |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884157/ https://www.ncbi.nlm.nih.gov/pubmed/20556198 http://dx.doi.org/10.3410/B1-95 |
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