Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays

High-throughput genotype data can be used to identify genes important for local adaptation in wild populations, phenotypes in lab stocks, or disease-related traits in human medicine. Here we advance microarray-based genotyping for population genomics with Restriction Site Tiling Analysis. The approa...

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Autores principales: Pespeni, Melissa H, Oliver, Thomas A, Manier, Mollie K, Palumbi, Stephen R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884547/
https://www.ncbi.nlm.nih.gov/pubmed/20403197
http://dx.doi.org/10.1186/gb-2010-11-4-r44
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author Pespeni, Melissa H
Oliver, Thomas A
Manier, Mollie K
Palumbi, Stephen R
author_facet Pespeni, Melissa H
Oliver, Thomas A
Manier, Mollie K
Palumbi, Stephen R
author_sort Pespeni, Melissa H
collection PubMed
description High-throughput genotype data can be used to identify genes important for local adaptation in wild populations, phenotypes in lab stocks, or disease-related traits in human medicine. Here we advance microarray-based genotyping for population genomics with Restriction Site Tiling Analysis. The approach simultaneously discovers polymorphisms and provides quantitative genotype data at 10,000s of loci. It is highly accurate and free from ascertainment bias. We apply the approach to uncover genomic differentiation in the purple sea urchin.
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spelling pubmed-28845472010-06-15 Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays Pespeni, Melissa H Oliver, Thomas A Manier, Mollie K Palumbi, Stephen R Genome Biol Method High-throughput genotype data can be used to identify genes important for local adaptation in wild populations, phenotypes in lab stocks, or disease-related traits in human medicine. Here we advance microarray-based genotyping for population genomics with Restriction Site Tiling Analysis. The approach simultaneously discovers polymorphisms and provides quantitative genotype data at 10,000s of loci. It is highly accurate and free from ascertainment bias. We apply the approach to uncover genomic differentiation in the purple sea urchin. BioMed Central 2010 2010-04-19 /pmc/articles/PMC2884547/ /pubmed/20403197 http://dx.doi.org/10.1186/gb-2010-11-4-r44 Text en Copyright ©2010 Pespeni et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Pespeni, Melissa H
Oliver, Thomas A
Manier, Mollie K
Palumbi, Stephen R
Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
title Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
title_full Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
title_fullStr Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
title_full_unstemmed Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
title_short Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
title_sort restriction site tiling analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884547/
https://www.ncbi.nlm.nih.gov/pubmed/20403197
http://dx.doi.org/10.1186/gb-2010-11-4-r44
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AT maniermolliek restrictionsitetilinganalysisaccuratediscoveryandquantitativegenotypingofgenomewidepolymorphismsusingnucleotidearrays
AT palumbistephenr restrictionsitetilinganalysisaccuratediscoveryandquantitativegenotypingofgenomewidepolymorphismsusingnucleotidearrays

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