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Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus

Human metapneumovirus (hMPV) is genetically related to respiratory syncytial virus (RSV); both cause respiratory tract illnesses ranging from a mild cough to bronchiolitis and pneumonia. The F protein-directed monoclonal antibody (mAb) palivizumab has been shown to prevent severe lower respiratory t...

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Autores principales: Ulbrandt, Nancy D., Ji, Hong, Patel, Nita K., Barnes, Arnita S., Wilson, Susan, Kiener, Peter A., Suzich, JoAnn, McCarthy, Michael P.
Formato: Texto
Lenguaje:English
Publicado: Society for General Microbiology 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885031/
https://www.ncbi.nlm.nih.gov/pubmed/19008400
http://dx.doi.org/10.1099/vir.0.2008/005199-0
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author Ulbrandt, Nancy D.
Ji, Hong
Patel, Nita K.
Barnes, Arnita S.
Wilson, Susan
Kiener, Peter A.
Suzich, JoAnn
McCarthy, Michael P.
author_facet Ulbrandt, Nancy D.
Ji, Hong
Patel, Nita K.
Barnes, Arnita S.
Wilson, Susan
Kiener, Peter A.
Suzich, JoAnn
McCarthy, Michael P.
author_sort Ulbrandt, Nancy D.
collection PubMed
description Human metapneumovirus (hMPV) is genetically related to respiratory syncytial virus (RSV); both cause respiratory tract illnesses ranging from a mild cough to bronchiolitis and pneumonia. The F protein-directed monoclonal antibody (mAb) palivizumab has been shown to prevent severe lower respiratory tract RSV infection in animals and humans. We have previously reported on a panel of mAbs against the hMPV F protein that neutralize hMPV in vitro and, in two cases, in vivo. Here we describe the generation of hMPV mAb-resistant mutants (MARMs) to these neutralizing antibodies. Sequencing the F proteins of the hMPV MARMs identified several neutralizing epitopes. Interestingly, some of the epitopes mapped on the hMPV F protein coincide with homologous regions mapped previously on the RSV F protein, including the site against which the broadly protective mAb palivizumab is directed. This suggests that these homologous regions play important, conserved functions in both viruses.
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spelling pubmed-28850312010-07-06 Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus Ulbrandt, Nancy D. Ji, Hong Patel, Nita K. Barnes, Arnita S. Wilson, Susan Kiener, Peter A. Suzich, JoAnn McCarthy, Michael P. J Gen Virol Animal Human metapneumovirus (hMPV) is genetically related to respiratory syncytial virus (RSV); both cause respiratory tract illnesses ranging from a mild cough to bronchiolitis and pneumonia. The F protein-directed monoclonal antibody (mAb) palivizumab has been shown to prevent severe lower respiratory tract RSV infection in animals and humans. We have previously reported on a panel of mAbs against the hMPV F protein that neutralize hMPV in vitro and, in two cases, in vivo. Here we describe the generation of hMPV mAb-resistant mutants (MARMs) to these neutralizing antibodies. Sequencing the F proteins of the hMPV MARMs identified several neutralizing epitopes. Interestingly, some of the epitopes mapped on the hMPV F protein coincide with homologous regions mapped previously on the RSV F protein, including the site against which the broadly protective mAb palivizumab is directed. This suggests that these homologous regions play important, conserved functions in both viruses. Society for General Microbiology 2008-12 /pmc/articles/PMC2885031/ /pubmed/19008400 http://dx.doi.org/10.1099/vir.0.2008/005199-0 Text en Copyright © 2008, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Animal
Ulbrandt, Nancy D.
Ji, Hong
Patel, Nita K.
Barnes, Arnita S.
Wilson, Susan
Kiener, Peter A.
Suzich, JoAnn
McCarthy, Michael P.
Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
title Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
title_full Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
title_fullStr Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
title_full_unstemmed Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
title_short Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
title_sort identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885031/
https://www.ncbi.nlm.nih.gov/pubmed/19008400
http://dx.doi.org/10.1099/vir.0.2008/005199-0
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