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Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda

The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies...

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Autores principales: Cook, Shelley, Moureau, Gregory, Harbach, Ralph E., Mukwaya, Louis, Goodger, Kim, Ssenfuka, Fred, Gould, Ernest, Holmes, Edward C., de Lamballerie, Xavier
Formato: Texto
Lenguaje:English
Publicado: Society for General Microbiology 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885038/
https://www.ncbi.nlm.nih.gov/pubmed/19656970
http://dx.doi.org/10.1099/vir.0.014183-0
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author Cook, Shelley
Moureau, Gregory
Harbach, Ralph E.
Mukwaya, Louis
Goodger, Kim
Ssenfuka, Fred
Gould, Ernest
Holmes, Edward C.
de Lamballerie, Xavier
author_facet Cook, Shelley
Moureau, Gregory
Harbach, Ralph E.
Mukwaya, Louis
Goodger, Kim
Ssenfuka, Fred
Gould, Ernest
Holmes, Edward C.
de Lamballerie, Xavier
author_sort Cook, Shelley
collection PubMed
description The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known ‘insect-only’ flavivirus, appearing to replicate in insect cells alone. A second member of the ‘insect-only’ group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the ‘insect-only’ group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel ‘insect-only’ flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa.
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spelling pubmed-28850382010-07-06 Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda Cook, Shelley Moureau, Gregory Harbach, Ralph E. Mukwaya, Louis Goodger, Kim Ssenfuka, Fred Gould, Ernest Holmes, Edward C. de Lamballerie, Xavier J Gen Virol Animal The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known ‘insect-only’ flavivirus, appearing to replicate in insect cells alone. A second member of the ‘insect-only’ group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the ‘insect-only’ group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel ‘insect-only’ flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa. Society for General Microbiology 2009-11 /pmc/articles/PMC2885038/ /pubmed/19656970 http://dx.doi.org/10.1099/vir.0.014183-0 Text en Copyright © 2009, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Animal
Cook, Shelley
Moureau, Gregory
Harbach, Ralph E.
Mukwaya, Louis
Goodger, Kim
Ssenfuka, Fred
Gould, Ernest
Holmes, Edward C.
de Lamballerie, Xavier
Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
title Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
title_full Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
title_fullStr Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
title_full_unstemmed Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
title_short Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
title_sort isolation of a novel species of flavivirus and a new strain of culex flavivirus (flaviviridae) from a natural mosquito population in uganda
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885038/
https://www.ncbi.nlm.nih.gov/pubmed/19656970
http://dx.doi.org/10.1099/vir.0.014183-0
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