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Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Society for General Microbiology
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885038/ https://www.ncbi.nlm.nih.gov/pubmed/19656970 http://dx.doi.org/10.1099/vir.0.014183-0 |
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author | Cook, Shelley Moureau, Gregory Harbach, Ralph E. Mukwaya, Louis Goodger, Kim Ssenfuka, Fred Gould, Ernest Holmes, Edward C. de Lamballerie, Xavier |
author_facet | Cook, Shelley Moureau, Gregory Harbach, Ralph E. Mukwaya, Louis Goodger, Kim Ssenfuka, Fred Gould, Ernest Holmes, Edward C. de Lamballerie, Xavier |
author_sort | Cook, Shelley |
collection | PubMed |
description | The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known ‘insect-only’ flavivirus, appearing to replicate in insect cells alone. A second member of the ‘insect-only’ group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the ‘insect-only’ group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel ‘insect-only’ flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa. |
format | Text |
id | pubmed-2885038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Society for General Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28850382010-07-06 Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda Cook, Shelley Moureau, Gregory Harbach, Ralph E. Mukwaya, Louis Goodger, Kim Ssenfuka, Fred Gould, Ernest Holmes, Edward C. de Lamballerie, Xavier J Gen Virol Animal The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known ‘insect-only’ flavivirus, appearing to replicate in insect cells alone. A second member of the ‘insect-only’ group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the ‘insect-only’ group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel ‘insect-only’ flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa. Society for General Microbiology 2009-11 /pmc/articles/PMC2885038/ /pubmed/19656970 http://dx.doi.org/10.1099/vir.0.014183-0 Text en Copyright © 2009, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Animal Cook, Shelley Moureau, Gregory Harbach, Ralph E. Mukwaya, Louis Goodger, Kim Ssenfuka, Fred Gould, Ernest Holmes, Edward C. de Lamballerie, Xavier Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda |
title | Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda |
title_full | Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda |
title_fullStr | Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda |
title_full_unstemmed | Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda |
title_short | Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda |
title_sort | isolation of a novel species of flavivirus and a new strain of culex flavivirus (flaviviridae) from a natural mosquito population in uganda |
topic | Animal |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885038/ https://www.ncbi.nlm.nih.gov/pubmed/19656970 http://dx.doi.org/10.1099/vir.0.014183-0 |
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