Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells

In this study, 4′,7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, (1)H and (13)C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce t...

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Detalles Bibliográficos
Autores principales: Xu, Keyong, Liu, Feng, Liu, Benguo, Gao, Han, Ning, Zhengxiang
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885088/
https://www.ncbi.nlm.nih.gov/pubmed/20559496
http://dx.doi.org/10.3390/ijms11051991
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author Xu, Keyong
Liu, Feng
Liu, Benguo
Gao, Han
Ning, Zhengxiang
author_facet Xu, Keyong
Liu, Feng
Liu, Benguo
Gao, Han
Ning, Zhengxiang
author_sort Xu, Keyong
collection PubMed
description In this study, 4′,7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, (1)H and (13)C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4′,7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4′,7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.
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spelling pubmed-28850882010-06-17 Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells Xu, Keyong Liu, Feng Liu, Benguo Gao, Han Ning, Zhengxiang Int J Mol Sci Communication In this study, 4′,7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, (1)H and (13)C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4′,7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4′,7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle. Molecular Diversity Preservation International (MDPI) 2010-04-30 /pmc/articles/PMC2885088/ /pubmed/20559496 http://dx.doi.org/10.3390/ijms11051991 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Xu, Keyong
Liu, Feng
Liu, Benguo
Gao, Han
Ning, Zhengxiang
Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells
title Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells
title_full Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells
title_fullStr Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells
title_full_unstemmed Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells
title_short Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells
title_sort synthesis of 4′,7-diacetoxyapigenin and its apoptotic induction in human hep g2 cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885088/
https://www.ncbi.nlm.nih.gov/pubmed/20559496
http://dx.doi.org/10.3390/ijms11051991
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AT gaohan synthesisof47diacetoxyapigeninanditsapoptoticinductioninhumanhepg2cells
AT ningzhengxiang synthesisof47diacetoxyapigeninanditsapoptoticinductioninhumanhepg2cells

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