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The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production

Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential,...

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Detalles Bibliográficos
Autores principales: Aramwit, Pornanong, Kanokpanont, Sorada, Nakpheng, Titpawan, Srichana, Teerapol
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885102/
https://www.ncbi.nlm.nih.gov/pubmed/20559510
http://dx.doi.org/10.3390/ijms11052200
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author Aramwit, Pornanong
Kanokpanont, Sorada
Nakpheng, Titpawan
Srichana, Teerapol
author_facet Aramwit, Pornanong
Kanokpanont, Sorada
Nakpheng, Titpawan
Srichana, Teerapol
author_sort Aramwit, Pornanong
collection PubMed
description Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content. The MTT assay indicated that SS from all extraction methods had no toxicity to mouse fibroblast cells at concentrations up to 40 μg/mL after 24 h incubation, but SS obtained from some extraction methods can be toxic at higher concentrations. Heat-degraded SS was the least toxic to cells and activated the highest collagen production, while urea-extracted SS showed the lowest cell viability and collagen production. SS from urea extraction was severely harmful to cells at concentrations higher than 100 μg/mL. SS from all extraction methods could still promote collagen production in a concentration-dependent manner, even at high concentrations that are toxic to cells.
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spelling pubmed-28851022010-06-17 The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production Aramwit, Pornanong Kanokpanont, Sorada Nakpheng, Titpawan Srichana, Teerapol Int J Mol Sci Article Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content. The MTT assay indicated that SS from all extraction methods had no toxicity to mouse fibroblast cells at concentrations up to 40 μg/mL after 24 h incubation, but SS obtained from some extraction methods can be toxic at higher concentrations. Heat-degraded SS was the least toxic to cells and activated the highest collagen production, while urea-extracted SS showed the lowest cell viability and collagen production. SS from urea extraction was severely harmful to cells at concentrations higher than 100 μg/mL. SS from all extraction methods could still promote collagen production in a concentration-dependent manner, even at high concentrations that are toxic to cells. Molecular Diversity Preservation International (MDPI) 2010-05-20 /pmc/articles/PMC2885102/ /pubmed/20559510 http://dx.doi.org/10.3390/ijms11052200 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Aramwit, Pornanong
Kanokpanont, Sorada
Nakpheng, Titpawan
Srichana, Teerapol
The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
title The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
title_full The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
title_fullStr The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
title_full_unstemmed The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
title_short The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
title_sort effect of sericin from various extraction methods on cell viability and collagen production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885102/
https://www.ncbi.nlm.nih.gov/pubmed/20559510
http://dx.doi.org/10.3390/ijms11052200
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