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The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential,...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885102/ https://www.ncbi.nlm.nih.gov/pubmed/20559510 http://dx.doi.org/10.3390/ijms11052200 |
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author | Aramwit, Pornanong Kanokpanont, Sorada Nakpheng, Titpawan Srichana, Teerapol |
author_facet | Aramwit, Pornanong Kanokpanont, Sorada Nakpheng, Titpawan Srichana, Teerapol |
author_sort | Aramwit, Pornanong |
collection | PubMed |
description | Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content. The MTT assay indicated that SS from all extraction methods had no toxicity to mouse fibroblast cells at concentrations up to 40 μg/mL after 24 h incubation, but SS obtained from some extraction methods can be toxic at higher concentrations. Heat-degraded SS was the least toxic to cells and activated the highest collagen production, while urea-extracted SS showed the lowest cell viability and collagen production. SS from urea extraction was severely harmful to cells at concentrations higher than 100 μg/mL. SS from all extraction methods could still promote collagen production in a concentration-dependent manner, even at high concentrations that are toxic to cells. |
format | Text |
id | pubmed-2885102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-28851022010-06-17 The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production Aramwit, Pornanong Kanokpanont, Sorada Nakpheng, Titpawan Srichana, Teerapol Int J Mol Sci Article Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content. The MTT assay indicated that SS from all extraction methods had no toxicity to mouse fibroblast cells at concentrations up to 40 μg/mL after 24 h incubation, but SS obtained from some extraction methods can be toxic at higher concentrations. Heat-degraded SS was the least toxic to cells and activated the highest collagen production, while urea-extracted SS showed the lowest cell viability and collagen production. SS from urea extraction was severely harmful to cells at concentrations higher than 100 μg/mL. SS from all extraction methods could still promote collagen production in a concentration-dependent manner, even at high concentrations that are toxic to cells. Molecular Diversity Preservation International (MDPI) 2010-05-20 /pmc/articles/PMC2885102/ /pubmed/20559510 http://dx.doi.org/10.3390/ijms11052200 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Aramwit, Pornanong Kanokpanont, Sorada Nakpheng, Titpawan Srichana, Teerapol The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production |
title | The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production |
title_full | The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production |
title_fullStr | The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production |
title_full_unstemmed | The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production |
title_short | The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production |
title_sort | effect of sericin from various extraction methods on cell viability and collagen production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885102/ https://www.ncbi.nlm.nih.gov/pubmed/20559510 http://dx.doi.org/10.3390/ijms11052200 |
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