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Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation

BACKGROUND: Many diseases associated with bone marrow transplantation (BMT) are caused by transplanted hematopoietic cells, and the onset of these diseases occurs after homing of donor cells in the initial phase after BMT. Noninvasive observation of donor cell homing shortly after transplantation is...

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Autores principales: Ushiki, Takashi, Kizaka-Kondoh, Shinae, Ashihara, Eishi, Tanaka, Shotaro, Masuko, Masayoshi, Hirai, Hideyo, Kimura, Shinya, Aizawa, Yoshifusa, Maekawa, Taira, Hiraoka, Masahiro
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885427/
https://www.ncbi.nlm.nih.gov/pubmed/20559437
http://dx.doi.org/10.1371/journal.pone.0011114
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author Ushiki, Takashi
Kizaka-Kondoh, Shinae
Ashihara, Eishi
Tanaka, Shotaro
Masuko, Masayoshi
Hirai, Hideyo
Kimura, Shinya
Aizawa, Yoshifusa
Maekawa, Taira
Hiraoka, Masahiro
author_facet Ushiki, Takashi
Kizaka-Kondoh, Shinae
Ashihara, Eishi
Tanaka, Shotaro
Masuko, Masayoshi
Hirai, Hideyo
Kimura, Shinya
Aizawa, Yoshifusa
Maekawa, Taira
Hiraoka, Masahiro
author_sort Ushiki, Takashi
collection PubMed
description BACKGROUND: Many diseases associated with bone marrow transplantation (BMT) are caused by transplanted hematopoietic cells, and the onset of these diseases occurs after homing of donor cells in the initial phase after BMT. Noninvasive observation of donor cell homing shortly after transplantation is potentially valuable for improving therapeutic outcomes of BMT by diagnosing the early stages of these diseases. METHODOLOGY/PRINCIPAL FINDINGS: Freshly harvested near-infrared fluorescence-labeled cells were noninvasively observed for 24 h after BMT using a photon counting device to track their homing process. In a congenic BMT model, the homing of Alexa Fluor 750-labeled donor cells in the tibia was detected less than 1 h after BMT. In addition, subsequent cell distribution in an intraBM BMT model was successfully monitored for the first time using this method. In the allogeneic BMT model, T-cell depletion decreased the near-infrared fluorescence (NIRF) signals of the reticuloendothelial system. CONCLUSIONS/SIGNIFICANCE: This approach in several murine BMT models revealed that the transplanted cells homed within 24 h after transplantation. NIRF labeling is useful for tracking transplanted cells in the initial phase after BMT, and this approach can contribute to in vivo studies aimed at improving the therapeutic outcomes of BMT.
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spelling pubmed-28854272010-06-17 Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation Ushiki, Takashi Kizaka-Kondoh, Shinae Ashihara, Eishi Tanaka, Shotaro Masuko, Masayoshi Hirai, Hideyo Kimura, Shinya Aizawa, Yoshifusa Maekawa, Taira Hiraoka, Masahiro PLoS One Research Article BACKGROUND: Many diseases associated with bone marrow transplantation (BMT) are caused by transplanted hematopoietic cells, and the onset of these diseases occurs after homing of donor cells in the initial phase after BMT. Noninvasive observation of donor cell homing shortly after transplantation is potentially valuable for improving therapeutic outcomes of BMT by diagnosing the early stages of these diseases. METHODOLOGY/PRINCIPAL FINDINGS: Freshly harvested near-infrared fluorescence-labeled cells were noninvasively observed for 24 h after BMT using a photon counting device to track their homing process. In a congenic BMT model, the homing of Alexa Fluor 750-labeled donor cells in the tibia was detected less than 1 h after BMT. In addition, subsequent cell distribution in an intraBM BMT model was successfully monitored for the first time using this method. In the allogeneic BMT model, T-cell depletion decreased the near-infrared fluorescence (NIRF) signals of the reticuloendothelial system. CONCLUSIONS/SIGNIFICANCE: This approach in several murine BMT models revealed that the transplanted cells homed within 24 h after transplantation. NIRF labeling is useful for tracking transplanted cells in the initial phase after BMT, and this approach can contribute to in vivo studies aimed at improving the therapeutic outcomes of BMT. Public Library of Science 2010-06-14 /pmc/articles/PMC2885427/ /pubmed/20559437 http://dx.doi.org/10.1371/journal.pone.0011114 Text en Ushiki et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ushiki, Takashi
Kizaka-Kondoh, Shinae
Ashihara, Eishi
Tanaka, Shotaro
Masuko, Masayoshi
Hirai, Hideyo
Kimura, Shinya
Aizawa, Yoshifusa
Maekawa, Taira
Hiraoka, Masahiro
Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation
title Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation
title_full Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation
title_fullStr Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation
title_full_unstemmed Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation
title_short Noninvasive Tracking of Donor Cell Homing by Near-Infrared Fluorescence Imaging Shortly after Bone Marrow Transplantation
title_sort noninvasive tracking of donor cell homing by near-infrared fluorescence imaging shortly after bone marrow transplantation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885427/
https://www.ncbi.nlm.nih.gov/pubmed/20559437
http://dx.doi.org/10.1371/journal.pone.0011114
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