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Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe

The multivesicular body (MVB) sorting pathway is required for a number of biological processes, including downregulation of cell-surface proteins and protein sorting into the vacuolar lumen. The function of this pathway requires endosomal sorting complexes required for transport (ESCRT) composed of...

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Autores principales: Iwaki, Tomoko, Onishi, Masayuki, Ikeuchi, Masaru, Kita, Ayako, Sugiura, Reiko, Giga-Hama, Yuko, Fukui, Yasuhisa, Takegawa, Kaoru
Formato: Texto
Lenguaje:English
Publicado: Microbiology Society 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885615/
https://www.ncbi.nlm.nih.gov/pubmed/17660439
http://dx.doi.org/10.1099/mic.0.2007/006072-0
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author Iwaki, Tomoko
Onishi, Masayuki
Ikeuchi, Masaru
Kita, Ayako
Sugiura, Reiko
Giga-Hama, Yuko
Fukui, Yasuhisa
Takegawa, Kaoru
author_facet Iwaki, Tomoko
Onishi, Masayuki
Ikeuchi, Masaru
Kita, Ayako
Sugiura, Reiko
Giga-Hama, Yuko
Fukui, Yasuhisa
Takegawa, Kaoru
author_sort Iwaki, Tomoko
collection PubMed
description The multivesicular body (MVB) sorting pathway is required for a number of biological processes, including downregulation of cell-surface proteins and protein sorting into the vacuolar lumen. The function of this pathway requires endosomal sorting complexes required for transport (ESCRT) composed of class E vacuolar protein sorting (Vps) proteins in Saccharomyces cerevisiae, many of which are conserved in Schizosaccharomyces pombe. Of these, sst4/vps27 (homologous to VPS27) and sst6 (similar to VPS23) have been identified as suppressors of sterility in ste12Δ (sst), although their functions have not been uncovered to date. In this report, these two sst genes are shown to be required for vacuolar sorting of carboxypeptidase Y (CPY) and an MVB marker, the ubiquitin–GFP–carboxypeptidase S (Ub–GFP–CPS) fusion protein, despite the lack of the ubiquitin E2 variant domain in Sst6p. Disruption mutants of a variety of other class E vps homologues also had defects in sorting of CPY and Ub–GFP–CPS. Sch. pombe has a mammalian AMSH homologue, sst2. Phenotypic analyses suggested that Sst2p is a class E Vps protein. Taken together, these results suggest that sorting into multivesicular bodies is dependent on class E Vps proteins, including Sst2p, in Sch. pombe.
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spelling pubmed-28856152010-07-06 Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe Iwaki, Tomoko Onishi, Masayuki Ikeuchi, Masaru Kita, Ayako Sugiura, Reiko Giga-Hama, Yuko Fukui, Yasuhisa Takegawa, Kaoru Microbiology (Reading) Cell and Developmental Biology The multivesicular body (MVB) sorting pathway is required for a number of biological processes, including downregulation of cell-surface proteins and protein sorting into the vacuolar lumen. The function of this pathway requires endosomal sorting complexes required for transport (ESCRT) composed of class E vacuolar protein sorting (Vps) proteins in Saccharomyces cerevisiae, many of which are conserved in Schizosaccharomyces pombe. Of these, sst4/vps27 (homologous to VPS27) and sst6 (similar to VPS23) have been identified as suppressors of sterility in ste12Δ (sst), although their functions have not been uncovered to date. In this report, these two sst genes are shown to be required for vacuolar sorting of carboxypeptidase Y (CPY) and an MVB marker, the ubiquitin–GFP–carboxypeptidase S (Ub–GFP–CPS) fusion protein, despite the lack of the ubiquitin E2 variant domain in Sst6p. Disruption mutants of a variety of other class E vps homologues also had defects in sorting of CPY and Ub–GFP–CPS. Sch. pombe has a mammalian AMSH homologue, sst2. Phenotypic analyses suggested that Sst2p is a class E Vps protein. Taken together, these results suggest that sorting into multivesicular bodies is dependent on class E Vps proteins, including Sst2p, in Sch. pombe. Microbiology Society 2007-08 /pmc/articles/PMC2885615/ /pubmed/17660439 http://dx.doi.org/10.1099/mic.0.2007/006072-0 Text en Copyright © 2007, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cell and Developmental Biology
Iwaki, Tomoko
Onishi, Masayuki
Ikeuchi, Masaru
Kita, Ayako
Sugiura, Reiko
Giga-Hama, Yuko
Fukui, Yasuhisa
Takegawa, Kaoru
Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
title Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
title_full Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
title_fullStr Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
title_full_unstemmed Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
title_short Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
title_sort essential roles of class e vps proteins for sorting into multivesicular bodies in schizosaccharomyces pombe
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885615/
https://www.ncbi.nlm.nih.gov/pubmed/17660439
http://dx.doi.org/10.1099/mic.0.2007/006072-0
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