Cargando…

Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae

The homothallic ascomycete fungus Gibberella zeae (anamorph: Fusarium graminearum) is a major toxigenic plant pathogen that causes head blight disease on small-grain cereals. The fungus produces the mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in infected hosts, posing a threat to human and...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Hye-Young, Seo, Jeong-Ah, Kim, Jung-Eun, Han, Kap-Hoon, Shim, Won-Bo, Yun, Sung-Hwan, Lee, Yin-Won
Formato: Texto
Lenguaje:English
Publicado: Microbiology Society 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885625/
https://www.ncbi.nlm.nih.gov/pubmed/18227243
http://dx.doi.org/10.1099/mic.0.2007/012260-0
_version_ 1782182402465988608
author Yu, Hye-Young
Seo, Jeong-Ah
Kim, Jung-Eun
Han, Kap-Hoon
Shim, Won-Bo
Yun, Sung-Hwan
Lee, Yin-Won
author_facet Yu, Hye-Young
Seo, Jeong-Ah
Kim, Jung-Eun
Han, Kap-Hoon
Shim, Won-Bo
Yun, Sung-Hwan
Lee, Yin-Won
author_sort Yu, Hye-Young
collection PubMed
description The homothallic ascomycete fungus Gibberella zeae (anamorph: Fusarium graminearum) is a major toxigenic plant pathogen that causes head blight disease on small-grain cereals. The fungus produces the mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in infected hosts, posing a threat to human and animal health. Despite its agricultural and toxicological importance, the molecular mechanisms underlying its growth, development and virulence remain largely unknown. To better understand such mechanisms, we studied the heterotrimeric G proteins of G. zeae, which are known to control crucial signalling pathways that regulate various cellular and developmental responses in fungi. Three putative Gα subunits, GzGPA1, GzGPA2 and GzGPA3, and one Gβ subunit, GzGPB1, were identified in the F. graminearum genome. Deletion of GzGPA1, a homologue of the Aspergillus nidulans Gα gene fadA, resulted in female sterility and enhanced DON and ZEA production, suggesting that GzGPA1 is required for normal sexual reproduction and repression of toxin biosynthesis. The production of DON and ZEA was also enhanced in the GzGPB1 mutant, suggesting that both Gα GzGPA1 and Gβ GzGPB1 negatively control mycotoxin production. Deletion of GzGPA2, which encodes a Gα protein similar to A. nidulans GanB, caused reduced pathogenicity and increased chitin accumulation in the cell wall, implying that GzGPA2 has multiple functions. Our study shows that G. zeae heterotrimeric G protein subunits can regulate vegetative growth, sexual development, toxin production and pathogenicity.
format Text
id pubmed-2885625
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-28856252010-07-06 Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae Yu, Hye-Young Seo, Jeong-Ah Kim, Jung-Eun Han, Kap-Hoon Shim, Won-Bo Yun, Sung-Hwan Lee, Yin-Won Microbiology (Reading) Plant-Microbe Interactions The homothallic ascomycete fungus Gibberella zeae (anamorph: Fusarium graminearum) is a major toxigenic plant pathogen that causes head blight disease on small-grain cereals. The fungus produces the mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in infected hosts, posing a threat to human and animal health. Despite its agricultural and toxicological importance, the molecular mechanisms underlying its growth, development and virulence remain largely unknown. To better understand such mechanisms, we studied the heterotrimeric G proteins of G. zeae, which are known to control crucial signalling pathways that regulate various cellular and developmental responses in fungi. Three putative Gα subunits, GzGPA1, GzGPA2 and GzGPA3, and one Gβ subunit, GzGPB1, were identified in the F. graminearum genome. Deletion of GzGPA1, a homologue of the Aspergillus nidulans Gα gene fadA, resulted in female sterility and enhanced DON and ZEA production, suggesting that GzGPA1 is required for normal sexual reproduction and repression of toxin biosynthesis. The production of DON and ZEA was also enhanced in the GzGPB1 mutant, suggesting that both Gα GzGPA1 and Gβ GzGPB1 negatively control mycotoxin production. Deletion of GzGPA2, which encodes a Gα protein similar to A. nidulans GanB, caused reduced pathogenicity and increased chitin accumulation in the cell wall, implying that GzGPA2 has multiple functions. Our study shows that G. zeae heterotrimeric G protein subunits can regulate vegetative growth, sexual development, toxin production and pathogenicity. Microbiology Society 2008-02 /pmc/articles/PMC2885625/ /pubmed/18227243 http://dx.doi.org/10.1099/mic.0.2007/012260-0 Text en Copyright © 2008, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant-Microbe Interactions
Yu, Hye-Young
Seo, Jeong-Ah
Kim, Jung-Eun
Han, Kap-Hoon
Shim, Won-Bo
Yun, Sung-Hwan
Lee, Yin-Won
Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae
title Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae
title_full Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae
title_fullStr Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae
title_full_unstemmed Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae
title_short Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae
title_sort functional analyses of heterotrimeric g protein gα and gβ subunits in gibberella zeae
topic Plant-Microbe Interactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885625/
https://www.ncbi.nlm.nih.gov/pubmed/18227243
http://dx.doi.org/10.1099/mic.0.2007/012260-0
work_keys_str_mv AT yuhyeyoung functionalanalysesofheterotrimericgproteingaandgbsubunitsingibberellazeae
AT seojeongah functionalanalysesofheterotrimericgproteingaandgbsubunitsingibberellazeae
AT kimjungeun functionalanalysesofheterotrimericgproteingaandgbsubunitsingibberellazeae
AT hankaphoon functionalanalysesofheterotrimericgproteingaandgbsubunitsingibberellazeae
AT shimwonbo functionalanalysesofheterotrimericgproteingaandgbsubunitsingibberellazeae
AT yunsunghwan functionalanalysesofheterotrimericgproteingaandgbsubunitsingibberellazeae
AT leeyinwon functionalanalysesofheterotrimericgproteingaandgbsubunitsingibberellazeae