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Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein
The humoral response to hepatitis C virus (HCV) may contribute to controlling infection. We previously isolated human monoclonal antibodies to conformational epitopes on the HCV E2 glycoprotein. Here, we report on their ability to inhibit infection by retroviral pseudoparticles incorporating a panel...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Society for General Microbiology
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885755/ https://www.ncbi.nlm.nih.gov/pubmed/18272755 http://dx.doi.org/10.1099/vir.0.83386-0 |
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author | Owsianka, Ania M. Tarr, Alexander W. Keck, Zhen-Yong Li, Ta-Kai Witteveldt, Jeroen Adair, Richard Foung, Steven K. H. Ball, Jonathan K. Patel, Arvind H. |
author_facet | Owsianka, Ania M. Tarr, Alexander W. Keck, Zhen-Yong Li, Ta-Kai Witteveldt, Jeroen Adair, Richard Foung, Steven K. H. Ball, Jonathan K. Patel, Arvind H. |
author_sort | Owsianka, Ania M. |
collection | PubMed |
description | The humoral response to hepatitis C virus (HCV) may contribute to controlling infection. We previously isolated human monoclonal antibodies to conformational epitopes on the HCV E2 glycoprotein. Here, we report on their ability to inhibit infection by retroviral pseudoparticles incorporating a panel of full-length E1E2 clones representing the full spectrum of genotypes 1–6. We identified one antibody, CBH-5, that was capable of neutralizing every genotype tested. It also potently inhibited chimeric cell culture-infectious HCV, which had genotype 2b envelope proteins in a genotype 2a (JFH-1) background. Analysis using a panel of alanine-substitution mutants of HCV E2 revealed that the epitope of CBH-5 includes amino acid residues that are required for binding of E2 to CD81, a cellular receptor essential for virus entry. This suggests that CBH-5 inhibits HCV infection by competing directly with CD81 for a binding site on E2. |
format | Text |
id | pubmed-2885755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Society for General Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28857552010-07-06 Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein Owsianka, Ania M. Tarr, Alexander W. Keck, Zhen-Yong Li, Ta-Kai Witteveldt, Jeroen Adair, Richard Foung, Steven K. H. Ball, Jonathan K. Patel, Arvind H. J Gen Virol Animal The humoral response to hepatitis C virus (HCV) may contribute to controlling infection. We previously isolated human monoclonal antibodies to conformational epitopes on the HCV E2 glycoprotein. Here, we report on their ability to inhibit infection by retroviral pseudoparticles incorporating a panel of full-length E1E2 clones representing the full spectrum of genotypes 1–6. We identified one antibody, CBH-5, that was capable of neutralizing every genotype tested. It also potently inhibited chimeric cell culture-infectious HCV, which had genotype 2b envelope proteins in a genotype 2a (JFH-1) background. Analysis using a panel of alanine-substitution mutants of HCV E2 revealed that the epitope of CBH-5 includes amino acid residues that are required for binding of E2 to CD81, a cellular receptor essential for virus entry. This suggests that CBH-5 inhibits HCV infection by competing directly with CD81 for a binding site on E2. Society for General Microbiology 2008-03 /pmc/articles/PMC2885755/ /pubmed/18272755 http://dx.doi.org/10.1099/vir.0.83386-0 Text en Copyright © 2008, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Animal Owsianka, Ania M. Tarr, Alexander W. Keck, Zhen-Yong Li, Ta-Kai Witteveldt, Jeroen Adair, Richard Foung, Steven K. H. Ball, Jonathan K. Patel, Arvind H. Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein |
title | Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein |
title_full | Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein |
title_fullStr | Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein |
title_full_unstemmed | Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein |
title_short | Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein |
title_sort | broadly neutralizing human monoclonal antibodies to the hepatitis c virus e2 glycoprotein |
topic | Animal |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885755/ https://www.ncbi.nlm.nih.gov/pubmed/18272755 http://dx.doi.org/10.1099/vir.0.83386-0 |
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