Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor

BACKGROUND: Staphylococcal (or micrococcal) nuclease or thermonuclease (SNase or Nuc) is a naturally-secreted nucleic acid degrading enzyme that participates in Staphylococcus aureus spread in the infected host. Purified Nuc protein can be used as an exogenous reagent to clear cellular extracts and...

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Detalles Bibliográficos
Autores principales: Trémillon, Nicolas, Issaly, Nicolas, Mozo, Julien, Duvignau, Thomas, Ginisty, Hervé, Devic, Eric, Poquet, Isabelle
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887397/
https://www.ncbi.nlm.nih.gov/pubmed/20492646
http://dx.doi.org/10.1186/1475-2859-9-37

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