The novel HSV-1 U(S)5-1 RNA is transcribed off a domain encoding U(S )5, U(S )4, U(S )3, U(S )2 and α22

BACKGROUND: The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, Ori(S)1 and Ori(S)2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. RESULTS: We describe here a novel RNA designated U(S)5-1 that spans 4.5 kb of the unique-short (U(S)) region. The RNA initiates in U(S)5 and terminates in the α22 open reading frame. It is expressed antisense to U(S)5, U(S)4, U(S)3 and ICP22 mRNAs. This transcript is expressed with γ(2 )kinetics and has a half-life of 80 minutes. CONCLUSION: These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA.