Cargando…

A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation

BACKGROUND: DNA methylation changes are widely used as early molecular markers in cancer detection. Sensitive detection and classification of rare methylation changes in DNA extracted from circulating body fluids or complex tissue samples is crucial for the understanding of tumor etiology, clinical...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tierling, Sascha, Schuster, Matthias, Tetzner, Reimo, Walter, Jörn
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887863/ https://www.ncbi.nlm.nih.gov/pubmed/20525169 http://dx.doi.org/10.1186/1756-8935-3-12

Ejemplares similares

Control of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA
por: Tetzner, Reimo, et al.
Publicado: (2007)

Comprehensive Evaluation of Commercial Bisulfite-Based DNA Methylation Kits and Development of an Alternative Protocol With Improved Conversion Performance
por: Tierling, Sascha, et al.
Publicado: (2018)

Validation of the SHOX2/PTGER4 DNA Methylation Marker Panel for Plasma-Based Discrimination between Patients with Malignant and Nonmalignant Lung Disease
por: Weiss, Gunter, et al.
Publicado: (2017)

The use of Multiple Displacement Amplified DNA as a control for Methylation Specific PCR, Pyrosequencing, Bisulfite Sequencing and Methylation-Sensitive Restriction Enzyme PCR
por: Hughes, Simon, et al.
Publicado: (2007)

Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA
por: Paraskevis, Dimitrios, et al.
Publicado: (2010)

CpG Island Methylation in Human Lymphocytes Is Highly Correlated with DNA Sequence, Repeats, and Predicted DNA Structure
por: Bock, Christoph, et al.
Publicado: (2006)

An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody
por: Kabir, M Enamul, et al.
Publicado: (2009)

Comparative Study of Autophagy in Oxaliplatin-Sensitive and Resistant SNU-C5 Colon Cancer Cells
por: Boo, Sun-Jin, et al.
Publicado: (2022)

Aflibercept Suppression of Angiopoietin-2 in a Rabbit Retinal Vascular Hyperpermeability Model
por: Lange, Claudia, et al.
Publicado: (2023)

Aflibercept Suppression of Angiopoietin-2 in a Rabbit Retinal Vascular Hyperpermeability Model
por: Lange, Claudia, et al.
Publicado: (2023)

Technical considerations in PCR-based assay design for diagnostic DNA methylation cancer biomarkers
por: Massen, Maartje, et al.
Publicado: (2022)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Genomic and probiotic characterization of SJP-SNU strain of Pichia kudriavzevii
por: Hong, Seung-Min, et al.
Publicado: (2018)

A robust internal control for high-precision DNA methylation analyses by droplet digital PCR
por: Pharo, Heidi D., et al.
Publicado: (2018)

Comprehensive evaluation of targeted multiplex bisulphite PCR sequencing for validation of DNA methylation biomarker panels
por: Lam, Dilys, et al.
Publicado: (2020)

Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
por: Varkonyi-Gasic, Erika, et al.
Publicado: (2007)

Prediction of smoking by multiplex bisulfite PCR with long amplicons considering allele-specific effects on DNA methylation
por: Kondratyev, Nikolay, et al.
Publicado: (2018)

A highly sensitive, PCR-based method for the detection of Plasmodium falciparum clones in microtiter plates
por: Maher, Steven P, et al.
Publicado: (2008)

PCR detection of malaria parasites and related haemosporidians: the sensitive methodology in determining bird-biting insects
por: Bernotienė, Rasa, et al.
Publicado: (2016)

Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR
por: Su, Yi, et al.
Publicado: (2018)

Establishment of Cell Lines from Both Myeloma Bone Marrow and Plasmacytoma: SNU_MM1393_BM and SNU_MM1393_SC from a Single Patient
por: Koh, Youngil, et al.
Publicado: (2014)

Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences
por: Candiloro, Ida L. M., et al.
Publicado: (2017)

Sensitive detection of BRAF V600E mutation by Amplification Refractory Mutation System (ARMS)-PCR
por: Huang, Tiangui, et al.
Publicado: (2013)

Volatilomic Signatures of AGS and SNU-1 Gastric Cancer Cell Lines
por: Ślefarska-Wolak, Daria, et al.
Publicado: (2022)

Array-based assay detects genome-wide 5-mC and 5-hmC in the brains of humans, non-human primates, and mice
por: Chopra, Pankaj, et al.
Publicado: (2014)

Adult monozygotic twins discordant for intra-uterine growth have indistinguishable genome-wide DNA methylation profiles
por: Souren, Nicole YP, et al.
Publicado: (2013)

Disagreement between two common biomarkers of global DNA methylation
por: Knothe, Claudia, et al.
Publicado: (2016)

SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR
por: Thompson, Christopher M., et al.
Publicado: (2015)

Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients
por: Deschaght, Pieter, et al.
Publicado: (2009)

Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B (p15) gene
por: Candiloro, Ida LM, et al.
Publicado: (2008)

Specific and sensitive detection of the conifer pathogen Gremmeniella abietina by nested PCR
por: Zeng, Qing-Yin, et al.
Publicado: (2005)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Digital PCR provides sensitive and absolute calibration for high throughput sequencing
por: White, Richard A, et al.
Publicado: (2009)

Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
por: Vestheim, Hege, et al.
Publicado: (2008)

Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses
por: Patel, Pranav, et al.
Publicado: (2013)

A novel, single-amplification PCR targeting mitochondrial genome highly sensitive and specific in diagnosing malaria among returned travellers in Bergen, Norway
por: Haanshuus, Christel G, et al.
Publicado: (2013)

Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples
por: Zhang, Eileen Z, et al.
Publicado: (2013)

Methyl-CpG-Binding PCR of Bloodspots for Confirmation of Fragile X Syndrome in Males
por: Tzeng, Ching-Cherng, et al.
Publicado: (2009)

An aging and p53 related marker: HOXA5 promoter methylation negatively correlates with mRNA and protein expression in old age
por: Feiner, Laura-Kim, et al.
Publicado: (2021)

Comparison of the effects of propofol and pentobarbital on hydrogen peroxide-stimulated hepatic SNU761 cells
por: Lee, Ji Yeon, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_5qtqe4o44peqcqdh6po4a1ib18