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Functional replacement of the endogenous tyrosyl-tRNA synthetase–tRNA(Tyr) pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion
Non-natural amino acids have been genetically encoded in living cells, using aminoacyl-tRNA synthetase–tRNA pairs orthogonal to the host translation system. In the present study, we engineered Escherichia coli cells with a translation system orthogonal to the E. coli tyrosyl-tRNA synthetase (TyrRS)–...
Autores principales: | Iraha, Fumie, Oki, Kenji, Kobayashi, Takatsugu, Ohno, Satoshi, Yokogawa, Takashi, Nishikawa, Kazuya, Yokoyama, Shigeyuki, Sakamoto, Kensaku |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887954/ https://www.ncbi.nlm.nih.gov/pubmed/20159998 http://dx.doi.org/10.1093/nar/gkq080 |
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