Cargando…
[C(7)GC(4)](4) Association into supra molecular i-motif structures
The self-associative properties of cytidine-rich oligonucleotides into symmetrical i-motif tetramers give to these oligonucleotides the capacity of forming supramolecular structures (sms) that have potential applications in the nanotechnology domain. In order to facilitate sms formation, oligonucleo...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887966/ https://www.ncbi.nlm.nih.gov/pubmed/20185569 http://dx.doi.org/10.1093/nar/gkq102 |
_version_ | 1782182619928068096 |
---|---|
author | Laisné, Aude Pompon, Denis Leroy, Jean-Louis |
author_facet | Laisné, Aude Pompon, Denis Leroy, Jean-Louis |
author_sort | Laisné, Aude |
collection | PubMed |
description | The self-associative properties of cytidine-rich oligonucleotides into symmetrical i-motif tetramers give to these oligonucleotides the capacity of forming supramolecular structures (sms) that have potential applications in the nanotechnology domain. In order to facilitate sms formation, oligonucleotides containing two cytidine stretches of unequal length (C(n)XC(m)) separated by a non-cytidine spacer were synthesized. They were designed to associate into a tetramer including an i-motif core built by intercalation of the C·C(+) pairs of the longer C stretch with the two dangling non-intercalated strands of the shorter C stretch at each end. Gel filtration chromatography shows that the non-intercalated C-rich ends give to this structure the capacity of forming extremely stable sms. Using C(7)GC(4) as a model, we find that the sms formation rate varies as the oligonucleotide concentration and increases at high temperature. Competitively with the tetramer involved in sms elongation, C(n)XC(m) oligonucleotides form i-motif dimers that compete with sms elongation. The dimer stability is strongly reduced when the pH is moved away from the cytidine pK. This results in an equilibrium shift towards the tetramer and in the acceleration of the sms formation rate. The chromatograms of the sms formed by C(7)GC(4) indicate a broad distribution. In a 1.5 mM solution incubated at 37°C, the equilibrium distribution is centered on a molecular weight corresponding to the assembly of nine tetramers and the upper limit corresponds to 80 tetramers. The lifetime of this structure is about 4 days at 40°C, pH 4.6. |
format | Text |
id | pubmed-2887966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28879662010-06-22 [C(7)GC(4)](4) Association into supra molecular i-motif structures Laisné, Aude Pompon, Denis Leroy, Jean-Louis Nucleic Acids Res Structural Biology The self-associative properties of cytidine-rich oligonucleotides into symmetrical i-motif tetramers give to these oligonucleotides the capacity of forming supramolecular structures (sms) that have potential applications in the nanotechnology domain. In order to facilitate sms formation, oligonucleotides containing two cytidine stretches of unequal length (C(n)XC(m)) separated by a non-cytidine spacer were synthesized. They were designed to associate into a tetramer including an i-motif core built by intercalation of the C·C(+) pairs of the longer C stretch with the two dangling non-intercalated strands of the shorter C stretch at each end. Gel filtration chromatography shows that the non-intercalated C-rich ends give to this structure the capacity of forming extremely stable sms. Using C(7)GC(4) as a model, we find that the sms formation rate varies as the oligonucleotide concentration and increases at high temperature. Competitively with the tetramer involved in sms elongation, C(n)XC(m) oligonucleotides form i-motif dimers that compete with sms elongation. The dimer stability is strongly reduced when the pH is moved away from the cytidine pK. This results in an equilibrium shift towards the tetramer and in the acceleration of the sms formation rate. The chromatograms of the sms formed by C(7)GC(4) indicate a broad distribution. In a 1.5 mM solution incubated at 37°C, the equilibrium distribution is centered on a molecular weight corresponding to the assembly of nine tetramers and the upper limit corresponds to 80 tetramers. The lifetime of this structure is about 4 days at 40°C, pH 4.6. Oxford University Press 2010-06 2010-02-25 /pmc/articles/PMC2887966/ /pubmed/20185569 http://dx.doi.org/10.1093/nar/gkq102 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Structural Biology Laisné, Aude Pompon, Denis Leroy, Jean-Louis [C(7)GC(4)](4) Association into supra molecular i-motif structures |
title | [C(7)GC(4)](4) Association into supra molecular i-motif structures |
title_full | [C(7)GC(4)](4) Association into supra molecular i-motif structures |
title_fullStr | [C(7)GC(4)](4) Association into supra molecular i-motif structures |
title_full_unstemmed | [C(7)GC(4)](4) Association into supra molecular i-motif structures |
title_short | [C(7)GC(4)](4) Association into supra molecular i-motif structures |
title_sort | [c(7)gc(4)](4) association into supra molecular i-motif structures |
topic | Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887966/ https://www.ncbi.nlm.nih.gov/pubmed/20185569 http://dx.doi.org/10.1093/nar/gkq102 |
work_keys_str_mv | AT laisneaude c7gc44associationintosupramolecularimotifstructures AT pompondenis c7gc44associationintosupramolecularimotifstructures AT leroyjeanlouis c7gc44associationintosupramolecularimotifstructures |