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Characterization of a novel wood mouse virus related to murid herpesvirus 4

Two novel gammaherpesviruses were isolated, one from a field vole (Microtus agrestis) and the other from wood mice (Apodemus sylvaticus). The genome of the latter, designated wood mouse herpesvirus (WMHV), was completely sequenced. WMHV had the same genome structure and predicted gene content as mur...

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Autores principales: Hughes, David J., Kipar, Anja, Milligan, Steven G., Cunningham, Charles, Sanders, Mandy, Quail, Michael A., Rajandream, Marie-Adele, Efstathiou, Stacey, Bowden, Rory J., Chastel, Claude, Bennett, Malcolm, Sample, Jeffery T., Barrell, Bart, Davison, Andrew J., Stewart, James P.
Formato: Texto
Lenguaje:English
Publicado: Society for General Microbiology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888160/
https://www.ncbi.nlm.nih.gov/pubmed/19940063
http://dx.doi.org/10.1099/vir.0.017327-0
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author Hughes, David J.
Kipar, Anja
Milligan, Steven G.
Cunningham, Charles
Sanders, Mandy
Quail, Michael A.
Rajandream, Marie-Adele
Efstathiou, Stacey
Bowden, Rory J.
Chastel, Claude
Bennett, Malcolm
Sample, Jeffery T.
Barrell, Bart
Davison, Andrew J.
Stewart, James P.
author_facet Hughes, David J.
Kipar, Anja
Milligan, Steven G.
Cunningham, Charles
Sanders, Mandy
Quail, Michael A.
Rajandream, Marie-Adele
Efstathiou, Stacey
Bowden, Rory J.
Chastel, Claude
Bennett, Malcolm
Sample, Jeffery T.
Barrell, Bart
Davison, Andrew J.
Stewart, James P.
author_sort Hughes, David J.
collection PubMed
description Two novel gammaherpesviruses were isolated, one from a field vole (Microtus agrestis) and the other from wood mice (Apodemus sylvaticus). The genome of the latter, designated wood mouse herpesvirus (WMHV), was completely sequenced. WMHV had the same genome structure and predicted gene content as murid herpesvirus 4 (MuHV4; murine gammaherpesvirus 68). Overall nucleotide sequence identity between WMHV and MuHV4 was 85 % and most of the 10 kb region at the left end of the unique region was particularly highly conserved, especially the viral tRNA-like sequences and the coding regions of genes M1 and M4. The partial sequence (71 913 bp) of another gammaherpesvirus, Brest herpesvirus (BRHV), which was isolated ostensibly from a white-toothed shrew (Crocidura russula), was also determined. The BRHV sequence was 99.2 % identical to the corresponding portion of the WMHV genome. Thus, WMHV and BRHV appeared to be strains of a new virus species. Biological characterization of WMHV indicated that it grew with similar kinetics to MuHV4 in cell culture. The pathogenesis of WMHV in wood mice was also extremely similar to that of MuHV4, except for the absence of inducible bronchus-associated lymphoid tissue at day 14 post-infection and a higher load of latently infected cells at 21 days post-infection.
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spelling pubmed-28881602011-03-30 Characterization of a novel wood mouse virus related to murid herpesvirus 4 Hughes, David J. Kipar, Anja Milligan, Steven G. Cunningham, Charles Sanders, Mandy Quail, Michael A. Rajandream, Marie-Adele Efstathiou, Stacey Bowden, Rory J. Chastel, Claude Bennett, Malcolm Sample, Jeffery T. Barrell, Bart Davison, Andrew J. Stewart, James P. J Gen Virol Animal Two novel gammaherpesviruses were isolated, one from a field vole (Microtus agrestis) and the other from wood mice (Apodemus sylvaticus). The genome of the latter, designated wood mouse herpesvirus (WMHV), was completely sequenced. WMHV had the same genome structure and predicted gene content as murid herpesvirus 4 (MuHV4; murine gammaherpesvirus 68). Overall nucleotide sequence identity between WMHV and MuHV4 was 85 % and most of the 10 kb region at the left end of the unique region was particularly highly conserved, especially the viral tRNA-like sequences and the coding regions of genes M1 and M4. The partial sequence (71 913 bp) of another gammaherpesvirus, Brest herpesvirus (BRHV), which was isolated ostensibly from a white-toothed shrew (Crocidura russula), was also determined. The BRHV sequence was 99.2 % identical to the corresponding portion of the WMHV genome. Thus, WMHV and BRHV appeared to be strains of a new virus species. Biological characterization of WMHV indicated that it grew with similar kinetics to MuHV4 in cell culture. The pathogenesis of WMHV in wood mice was also extremely similar to that of MuHV4, except for the absence of inducible bronchus-associated lymphoid tissue at day 14 post-infection and a higher load of latently infected cells at 21 days post-infection. Society for General Microbiology 2010-04 /pmc/articles/PMC2888160/ /pubmed/19940063 http://dx.doi.org/10.1099/vir.0.017327-0 Text en Copyright © 2010, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Animal
Hughes, David J.
Kipar, Anja
Milligan, Steven G.
Cunningham, Charles
Sanders, Mandy
Quail, Michael A.
Rajandream, Marie-Adele
Efstathiou, Stacey
Bowden, Rory J.
Chastel, Claude
Bennett, Malcolm
Sample, Jeffery T.
Barrell, Bart
Davison, Andrew J.
Stewart, James P.
Characterization of a novel wood mouse virus related to murid herpesvirus 4
title Characterization of a novel wood mouse virus related to murid herpesvirus 4
title_full Characterization of a novel wood mouse virus related to murid herpesvirus 4
title_fullStr Characterization of a novel wood mouse virus related to murid herpesvirus 4
title_full_unstemmed Characterization of a novel wood mouse virus related to murid herpesvirus 4
title_short Characterization of a novel wood mouse virus related to murid herpesvirus 4
title_sort characterization of a novel wood mouse virus related to murid herpesvirus 4
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888160/
https://www.ncbi.nlm.nih.gov/pubmed/19940063
http://dx.doi.org/10.1099/vir.0.017327-0
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